Pressure BioScience PBIO OTCQB Converts Debt To Equity -In Breakout Mode Target 400

1. Pressure BioScience – PBIO OTCQB – Converts Debt To Equity -In Breakout Mode – Target $4.00 thestreetreports.com/pressure-bioscience-pbio-otcqb-converts-debt-to-equity-in-breakout-mode-target-4-00 WALL STREET NEWS CEO Game Changer Interview BREAKOUT ALERT PRESSURE BIOSCIENCES INC OTC PBIO Converts $10 Mill Debt To Equity Revolutionize Edible Cannabis Industry China Orders 16 Units Investor Contact – Gary Zwetchkenbaum- Plum Tree Consulting LLC -Cell : 516 – 455-7662 – Email – gzplumtree@gmail.com Latest Video CEO Interview These shares are in BREAKOUT TERRITORY having rapidly advanced from $0.91 cents to $1.90 over the past weeks . We look for a target of $4.00 based on technical breakout above $2.00 1/17

2. We urge all serious investors to immediately place these shares on their Buy List ************************************** Pressure BioSciences Announces the Exchange of Over $10 Million of Debt into Equity Company’s Largest Investor Exchanges First Seniority Debt into Non-Secured Equity at 38% Above-Market Price, Expressing Confidence and Support SOUTH EASTON, MA / ACCESSWIRE / March 1, 2023 / Pressure BioSciences, Inc. (OTCQB:PBIO) (“PBIO” or the “Company”), a global leader in the development and sale of broadly enabling, high-pressure-based products and services to the life sciences and other industries, today announced that the Company’s largest shareholder has exchanged over $10 million of senior secured debt into unsecured preferred stock. The debt being converted is comprised of convertible promissory notes, accrued and unpaid interest on all promissory notes issued to the investor since November 2019, plus accrued and unpaid dividends on the Company’s Series AA Convertible Preferred Stock purchased by the investor since May 2018. The $10+ million of debt exchanged into equity represents over 50% of the total debt currently owed to the Investor by the Company and represents over 30% of the Company’s total outstanding debt held by all investors through February 2023 via (i) convertible promissory notes; (ii) accrued and unpaid interest on convertible promissory notes; and (iii) accrued and unpaid dividends earned from investments in the Company’s Series AA Convertible Preferred Stock financing. The exchange of debt to equity was consummated at a value of $2.50 per share of Common Stock, which is an approximate 38% premium over the Company’s recent open market valuation. The Company recently announced a number of significant achievements and collaborations, particularly related to its revolutionary Ultra Shear™ Technology (“UltraShear™”) platform for its “best-in-class” nanoemulsion processing. Among these are: (i) the development of “next generation” anti-aging skincare and hair regrowth products with partner Dr. Denese 2/17

3. SkinScience; (ii) the development of a sports performance/recovery drink with NBA legend Isiah Thomas’ One World Products (OTCQB: OWPO); and (iii) the release of dramatic consumer testing results, indicating that PBIO’s UltraShear platform is forging a game- changing future for the cannabis, nutraceuticals, and supplements industries. Mr. Richard T. Schumacher, President and CEO of PBIO, commented: “The PBIO Team is delighted with the strong recent progress in multiple aspects of our business. We are redesigning our production facilities, adding to our team, strengthening our core capabilities, filing additional UltraShear patents, building instrument inventories, and expanding our list of existing and prospective 2023 customers. We are engaging deeper into building strong strategic partnerships with key companies and individuals, including several billion-dollar companies who are global best-in-class players in priority markets targeted for explosive growth. In addition, we have begun forging stronger relationships with multiple strategic and retail investors.” Mr. Schumacher continued: “It has been a long time since I’ve seen and felt such excitement, enthusiasm, and confidence in the future of PBIO within our shareholder base. We believe this announced conversion of debt into equity is only the beginning of continued strengthening in our financial position and outlook. We are in discussions with other large shareholders who have expressed similar interest in supporting the Company through continuing improvements in our balance sheet, by exchanging additional millions of dollars of debt into equity, following the confidence and leadership demonstrated by the Company’s long-standing, largest investor. We anticipate additional similar announcements in the coming weeks.” Mr. Jeffrey N. Peterson, Chairman of PBIO, added: “The PBIO team has demonstrated tremendous dedication and execution in converting their vision and planning for our patent- protected nanoemulsions breakthrough technology into a proven reality that is already demonstrating the transformational power to restate the basis for competition in multiple multi-billion-dollar markets, spanning nutraceuticals, cosmeceuticals, pharmaceuticals, food and beverage, agrochemicals, and industrial markets. We are deeply appreciative for the patience and confidence demonstrated by our largest investor in this pivotal decision, and to all our investors who have delivered us to this inflection point, as we accelerate forward through commercialization, growth, capacity expansion, further capitalization, uplist to NASDAQ/NYSE, and our expected transition to profitability during the next year.” About Pressure BioSciences, Inc. Pressure BioSciences, Inc. (OTCQB: PBIO) is a leader in the development and sale of innovative, broadly enabling, pressure-based solutions for the worldwide life sciences and other industries. Our products are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or PCT) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus is in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, 3/17

4. forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technologies in the following areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. Forward Looking Statements This press release contains forward-looking statements. These statements relate to future events or our future financial performance and involve known and unknown risks, uncertainties and other factors that may cause our or our industry’s actual results, levels of activity, performance, or achievements to be materially different from any future results, levels of activity, performance or achievements expressed, implied, or inferred by these forward-looking statements. In some cases, you can identify forward-looking statements by terminology such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “intends,” “anticipates,” “believes,” estimates,” “predicts,” “projects,” “potential” or “continue” or the negative of such terms and other comparable terminology. These statements are only predictions based on our current expectations and projections about future events. You should not place undue reliance on these statements. In evaluating these statements, you should specifically consider various factors. Actual events or results may differ materially. These and other factors may cause our actual results to differ materially from any forward- looking statement. These risks, uncertainties, and other factors include, but are not limited to, the risks and uncertainties discussed under the heading “Risk Factors” in the Company’s Annual Report on Form 10-K for the year ended December 31, 2021, and other reports filed by the Company from time to time with the SEC. The Company undertakes no obligation to update any of the information included in this release, except as otherwise required by law. Pressure BioSciences’ Breakthrough UltraShear Nanoemulsion Processing Could Help Revolutionize The Edible Cannabis Industry Earlier this month, Pressure BioSciences, Inc. (OTCQB: PBIO) announced potentially ground-breaking results from a consumer focus group testing a THC oral spray developed using the company’s innovative UltraShear™ Technology nanoemulsification platform. The oral spray boasted strong potency and extremely fast absorption that could put it on track to become a best-in-class cannabis product in terms of speed to effects, stability, and bioavailability. The THC oral spray tested in this consumer focus group is being developed in partnership with California-based cannabis manufacturer, Crème De Canna. The news comes on the heels of the company’s December announcement of its first commercial shipment of a CBD oral spray manufactured with the same UltraShear technology. The $75,000 shipment to Safer Medical of Montana is the first of many shipments the company expects to make to distributors of CBD products throughout the 4/17

5. U.S. during 2023. In recent press releases, PBIO has stated that CBD products manufactured using its patented UltraShear platform could potentially yield many millions in revenue before the end of its 2023 fiscal year. WEBSITE PRODICTS TEAR SHEET SEC FILINGS PRESENTATION Slow And Inconsistent THC Absorption Remains A Key Challenge for Cannabis Manufacturers Because cannabinoids, like the well-known THC and CBD, are oil-soluble rather than water- soluble, the human body struggles to absorb them efficiently. As a consequence, much of the active ingredient in edible cannabis products is absorbed slowly, intermittently, or hardly at all. In one review of cannabis research, only 4% to 12% of THC was absorbed by the body when taken orally. Not only does that mean that the majority of the THC in an edible ends up getting wasted, but the variation in bioavailability between 4% and 12% is highly significant. A consumer eating the same edible product from the same manufacturer with the same dose might absorb just 4% one day but 12% the next day, leading to very different results. This makes it incredibly difficult for manufacturers to achieve precise dosing and effects in their edibles. It also increases production costs since they often need to include more than the minimum effective dose to compensate for the fact that most of the active ingredients won’t be absorbed. In addition to inefficient and inconsistent cannabinoid absorption, another challenge in the edible cannabis industry is absorption time. It is estimated to take anywhere from 30 minutes to two hours from the time a consumer ingests an edible to the time they start to feel its first effects, and then up to four hours to achieve peak effects. “Impatient consumers may repeatedly consume their edibles, only to be unhappily overdosed shortly thereafter,” explained PBI’s Director of Sales and Marketing, John Hollister in a statement on the consumer testing results. For patients using cannabis as a treatment for pain, anxiety, or depression, that delayed response also means edible cannabis is often unable to provide the instant relief they need. While smoking or vaping comes with a higher bioavailability of around 30% and a faster response time of seconds or minutes, the respiratory health consequences make it a less- than-ideal option, especially for regular use. PBI’s UltraShear Nanoemulsion Technology Could Be A Breakthrough In Achieving Precision Dosing for THC, CBD, and Other Products Consumers in the focus group reported that the first effects could be felt less than 10 minutes after initially using the innovative UltraShear processed THC oral spray, with many reporting noticeable effects within 1-5 minutes. Peak effects were achieved between 15-60 5/17

6. minutes after taking the dose. The results of this initial focus group could be a game-changer for an industry that’s been struggling with the challenges around consistent dosing and effective non-inhalation absorption. “This testing has provided dramatic consumer-level validation in the cannabis market for our UltraShear nanoemulsion capabilities and for its expected market-changing impact,” said Mr. Hollister. The UltraShear technology used to make the oral spray is PBI’s patented pressure-based platform that’s capable of creating what the company describes as the “best-in-class” nanoemulsions worldwide. In the cannabis nanoemulsion, the oil drops containing the CBD or THC molecules are sheared down to a nanosize so small that they are effectively water- soluble. This makes for a significantly more easily absorbed product that can be more precisely and accurately dosed. For consumers, that means more immediate and consistent effects every time they use an UltraShear processed oral spray or other mechanisms for ingestion. For manufacturers, that improved bioavailability means smaller amounts of THC or CBD are required to achieve an effective dose since the less active ingredient is getting wasted – thus saving the manufacturer money. “It provides an easy-to-use, reliable, and healthy alternative dosing format, with nearly immediate onset and a consistent experience associated with inhaling, but without the respiratory health risks associated with smoking and vaping. It also eliminates the frustrating delayed effects, imprecision, and frequent overdosing associated with edibles,” said Crème De Canna President, Jonathan Kolodinski. This article was originally published on Benzinga here . Pressure BioSciences, Inc. (OTCQB:PBIO) is a leader in the development and sale of innovative, broadly enabling, pressure-based solutions for the worldwide life sciences and other industries. Our products are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or PCT) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus is in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technologies in the following areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) and to (ii) prepare higher quality, homogenized, extended shelf- life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. 6/17

7. This press release contains forward-looking statements. These statements relate to future events or our future financial performance and involve known and unknown risks, uncertainties and other factors that may cause our or our industry’s actual results, levels of activity, performance, or achievements to be materially different from any future results, levels of activity, performance or achievements expressed, implied, or inferred by these forward-looking statements. In some cases, you can identify forward-looking statements by terminology such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “intends,” “anticipates,” “believes,” estimates,” “predicts,” “projects,” “potential” or “continue” or the negative of such terms and other comparable terminology. These statements are only predictions based on our current expectations and projections about future events. You should not place undue reliance on these statements. In evaluating these statements, you should specifically consider various factors. Actual events or results may differ materially. These and other factors may cause our actual results to differ materially from any forward-looking statement. These risks, uncertainties, and other factors include, but are not limited to, the risks and uncertainties discussed under the heading “Risk Factors” in the Company’s Annual Report on Form 10-K for the year ended December 31, 2021, and other reports filed by the Company from time to time with the SEC. The Company undertakes no obligation to update any of the information included in this release, except as otherwise required by law. This post contains sponsored advertising content. This content is for informational purposes only and is not intended to be investing advice. Pressure BioSciences Receives Record Nearly $600,000 Order for 16 PCT Instruments From Peoples Republic Of China Largest Single Order in Company’s History was Prepaid as New Chinese Distributor Comes Online; Adds to Revenue Strength Also Building Across PBI’s UltraShear Nanoemulsions Platform SOUTH EASTON, MA / ACCESSWIRE / February 1, 2023 / Pressure BioSciences, Inc. (OTCQB:PBIO) (“PBI” or the “Company”), a global leader in the development and sale of broadly enabling, high-pressure-based products and services to the life sciences and other industries, today announced entrance into an exclusive distribution agreement for the People’s Republic of China (“Agreement”) with PRS International Trade (Shanghai) Co., Ltd. (“PRS International”). Reflecting confidence built upon PBIO’s long investment in developing worldwide market support for the Company’s Pressure Cycling Technology (“PCT”) platform, PRS International prepaid for the entire purchase of 16 Barocycler EXT units for a total of nearly $600,000, with a commitment to purchase additional units and related consumables before the end of 2023. This historic purchase of PBIO’s original high-pressure technology instruments comes on the back of a surge in purchase orders for all product categories of the Company’s pressure-based instruments and consumables. 7/17

8. Director of Sales and Marketing John B. Hollister commented, “We believe this purchase of a record number of Barocycler EXT instruments, together with strong worldwide indications of purchase plans for additional non-PCT instruments and consumables, indicates that China and the rest of the life sciences world is finally emerging from under the economic cloud of COVID. We expect first quarter 2023 sales to benefit from the initial shipment of up to five Barocycler EXT instruments to China, from the surge in orders we are experiencing in our other pressure-based instrument areas (e.g., Barocyclers, HUB pressure generators, and cell disruptors), and from our recently opened UltraShear™ nanoemulsions tolling services. Adding to this excitement, we had a few expected sales in 2022 slip into 2023 due to supply chain issues. The net effect is that we are having a terrific first quarter, which we believe will continue to strengthen throughout 2023 and beyond.” Richard T. Schumacher, President and CEO, expanded: “We have been focusing the majority of our efforts on the launch of our revolutionary UltraShear nanoemulsions processing platform, which we expect will lead to ramping revenue in 2023. Additionally, our long-term investment in building broad-based market support for our line of pressure-based instruments continues to pay off with growing penetration and adoption for our initial high- pressure technology platforms and application areas. Our commitment to supporting this key area of our business remains unwavering. We are honored and humbled by the large, prepaid order from PRS International, and we are delighted to be in a position to reinvest our strengthening technical and financial foundation in support of our next generation UltraShear nanoemulsions technology platform that we believe will be manifesting strong commercial traction starting in this quarter.” Jeffrey N. Peterson, Chairman of PBIO, observed, “Ric and his team were visionaries in building strong scientific support and credibility in the Company’s initial PCT platform technology and applications areas. While we see market opportunities growing into the tens of millions of dollars for this platform in the coming years, our strategic focus has appropriately advanced onto our BaroFold and now our UltraShear platforms. BaroFold is an applications arena that we believe offers future potential growth that could be multiples greater for PBIO than the PCT product line. However, our investment in the development of the UltraShear™ platform, for the production of extremely stable and bioavailable nanoemulsions of oil-soluble active molecules for effective delivery into humans, animals, and plants, is a game-changing technology that we believe offers PBI opportunities into the hundreds of millions of dollars, if not more, as it re-defines the basis of competition in multiple multi-billion dollar industries, from pharmaceuticals to nutraceuticals, cosmeceuticals, food/beverage, agrochemicals, and more.” About Pressure BioSciences, Inc. Pressure BioSciences, Inc. (OTCQB:PBIO) is a leader in the development and sale of innovative, broadly enabling, pressure-based solutions for the worldwide life sciences and other industries. Our products are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or PCT) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus is in the development of PCT-based products for biomarker and target discovery, drug design and 8/17

9. development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technologies in the following areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. Forward Looking Statements This press release contains forward-looking statements. These statements relate to future events or our future financial performance and involve known and unknown risks, uncertainties and other factors that may cause our or our industry’s actual results, levels of activity, performance, or achievements to be materially different from any future results, levels of activity, performance or achievements expressed, implied, or inferred by these forward-looking statements. In some cases, you can identify forward-looking statements by terminology such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “intends,” “anticipates,” “believes,” estimates,” “predicts,” “projects,” “potential” or “continue” or the negative of such terms and other comparable terminology. These statements are only predictions based on our current expectations and projections about future events. You should not place undue reliance on these statements. In evaluating these statements, you should specifically consider various factors. Actual events or results may differ materially. These and other factors may cause our actual results to differ materially from any forward- looking statement. These risks, uncertainties, and other factors include, but are not limited to, the risks and uncertainties discussed under the heading “Risk Factors” in the Company’s Annual Report on Form 10-K for the year ended December 31, 2021, and other reports filed by the Company from time to time with the SEC. The Company undertakes no obligation to update any of the information included in this release, except as otherwise required by law. Pressure BioSciences and One World Products Announce Partnership in Development of CBD-Nanoemulsion Sports Performance/Recovery Drinks NBA Legend Isiah Thomas Leads Effort to Innovate and Deliver Next Generation Sports Drinks SOUTH EASTON, MA / ACCESSWIRE / January 27, 2023 / Pressure BioSciences, Inc. (OTCQB:PBIO) (“PBI” or the “Company”), a global leader in the development and sale of broadly enabling, high-pressure-based products and services to the life sciences industry, and One World Products, Inc. (OTCQB: OWPC) (“OWPC”), the largest Black-controlled, fully-licensed hemp and cannabis producer in Colombia, today announced they have entered into an active collaboration agreement (“Agreement”) under which the two companies will combine their strong, symbiotic capabilities to develop an innovative line of next generation sports performance/recovery drinks. 9/17

10. The Sports Performance/Recovery Drink Market encompasses live sports performance optimization, rapid and effective full recovery post-performance, and health and wellness areas – including immune health and antioxidant benefits. Fast delivery and uptake of therapeutic and nutritional ingredients must be made effective for the many oil-soluble active ingredients used in sports drinks. This is where PBIO’s revolutionary UltraShear™ nanoemulsions platform technology is already demonstrating game-changing results across multiple major consumer market applications. The “Recovery” market has also been supported by increasing numbers of consumers who consider themselves “non-athletes” joining in the sports drink craze. Data from IRI put sales of sports/performance beverages at about $9.5 billion for 2021 (sports drink market 2021). Isiah Thomas, Chairman and CEO of OWPC, highlighted: “This revolutionary new line of sports performance/recovery drinks will contain OWPC’s premium quality CBD that has been processed into a highly stable and extremely bioavailable nanoemulsion by PBIO’s patented UltraShear technology platform. This next generation CBD-nanoemulsion beverage is being designed for fast reduction of inflammation and pain, improved recovery time, and enhanced overall performance – all to be achieved quicker than any competing drink on the market today. It is being formulated to help athletes push through the tough moments of a workout or competition, and to recover more quickly afterward. In professional and even amateur sports, athletes understand the criticality of peak performance and recovery.” Mr. Thomas continued: “OWPC has been a highly sought after choice for the development of new and premium technologies for delivery of hemp and cannabis-derived products. I am excited that we have an opportunity to pair our premium quality CBD product with PBIO’s proprietary, cutting-edge nanoemulsification technology to deliver a uniquely-innovative and effective product to market that we believe will offer unmatched rapid absorption and full dosing of hard-to-deliver oil-soluble ingredients – to achieve game-changing performance.” Richard T. Schumacher, President and CEO of PBIO, expanded: “Partnering with Isiah and his exceptional team at OWPC is an extraordinary opportunity for PBIO to accelerate the visibility and impact of our revolutionary UltraShear nanoemulsions platform technology. Isiah is a world-renowned figure in the world of sports and more broadly as a role model in Black-owned business leadership, and it is a privilege for our companies to team up! OWPC has become a leading producer of the highest quality and sustainably sourced hemp-derived CBD in Colombia, and is closely partnered with their government and indigenous populations. Teamed with PBIO’s best-in-class nanoemulsions processing, OWPC is uniquely positioned to deliver these solutions and drive rapid recognition and acceptance across the worlds of professional and amateur sports. This is going to be exciting and, we believe, very successful!” About One World Products, Inc. One World Products (OTCQB: OWPC) is the largest Black-controlled, fully licensed hemp and cannabis producer with offices in Las Vegas, Nevada, and offices and operations in Bogota and Popayan, Colombia. One World Products planted its first crop of cannabis in 10/17

11. 2018 at its cultivation site in Popayan, Colombia, and began harvesting commercially in the first quarter of 2020. About Mr. Isiah Thomas Isiah Thomas was inducted into the National Basketball Association (NBA) Hall of Fame in 2000 after spending his entire career with the Detroit Pistons. He was a 12-time NBA All- Star, a two-time NBA champion, the 1990 NBA Finals Most Valuable Player, and named as one of the 50 Greatest Players in NBA History. Today, Mr. Thomas serves as an analyst for NBA TV and is an accomplished and highly respected international business executive and investor. His portfolio of companies was developed through Isiah International, Inc., which invests in companies with strong market positions and growth potential. About Pressure BioSciences, Inc. Pressure BioSciences, Inc. (OTCQB: PBIO) is a leader in the development and sale of innovative, broadly enabling, pressure-based solutions for the worldwide life sciences and other industries. Our products are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or PCT) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus is in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technologies in the following areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. Forward Looking Statements This press release contains forward-looking statements. These statements relate to future events or our future financial performance and involve known and unknown risks, uncertainties and other factors that may cause our or our industry’s actual results, levels of activity, performance, or achievements to be materially different from any future results, levels of activity, performance or achievements expressed, implied, or inferred by these forward-looking statements. In some cases, you can identify forward-looking statements by terminology such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “intends,” “anticipates,” “believes,” estimates,” “predicts,” “projects,” “potential” or “continue” or the negative of such terms and other comparable terminology. These statements are only predictions based on our current expectations and projections about future events. You should not place undue reliance on these statements. In evaluating these statements, you should specifically consider various factors. Actual events or results may differ materially. 11/17

12. These and other factors may cause our actual results to differ materially from any forward- looking statement. These risks, uncertainties, and other factors include, but are not limited to, the risks and uncertainties discussed under the heading “Risk Factors” in the Company’s Annual Report on Form 10-K for the year ended December 31, 2021, and other reports filed by the Company from time to time with the SEC. The Company undertakes no obligation to update any of the information included in this release, except as otherwise required by law. Pressure BioSciences Nanoemulsions Platform Forging Game-Changing Future for Cannabis Industry Dramatic Consumer Testing Results Confirm UltraShear Nanoemulsion THC Oral Spray Delivers First Effects and Maximization in Lightning Speed – Simple, Reliable Dosing Delivers Profoundly Improved Results SOUTH EASTON, MA / ACCESSWIRE / January 19, 2023 / Pressure BioSciences, Inc. (OTCQB:PBIO) (“PBI” or “Company”) today announced results from initial consumer focus group testing of a THC Nanoemulsion Oral Spray prepared using PBI’s revolutionary Ultra Shear Technology™ (UltraShear™ or UST™) platform. Preparation of the THC Nanoemulsion Oral Spray was completed by PBI’s California-based partner companies, including Crème De Canna (CdC), a CA-licensed manufacturer and long-established provider of innovative, high-quality cannabis products in CA. Mr. John B. Hollister, PBI’s Director of Sales and Marketing, explained: “Cannabinoids like THC are oil-soluble molecules that are typically poorly absorbed when ingested by our water-based human bodies. For THC edibles, most consumers report waiting 30-60 minutes for first effects and 60-90 minutes to peak effects. Impatient consumers may repeatedly consume their edibles, only to be unhappily over-dosed two hours later. Conversely, smoking and vaping produce first effects immediately and peak effects quickly but are increasingly in disfavor for the well-known respiratory health consequences that accumulate.” Mr. Hollister continued: “PBI’s partners in California used our revolutionary UltraShear platform to prepare extreme nanoemulsions of THC into an Oral Spray for consumer testing. Multiple test groups have confirmed that the UltraShear Nanoemulsion THC Oral Spray produces first effects between 1-10 minutes, often in the first 1-5 minutes, with peak effects reached in 15-60 minutes, often within 15-30 minutes. This testing has provided dramatic consumer-level validation in the cannabis market for our UltraShear nanoemulsion capabilities and for its expected market-changing impact. We are anticipating substantial revenues from the first THC UltraShear Nanoemulsion Oral Spray to be marketed by our California partners, beginning in this quarter.” Must See CEO VIDEO – Recent Podcast Mr. Jonathan Kolodinski, President of CdC, said: Crème De Canna is very excited to partner with PBI on the development of this groundbreaking dosing modality that will disrupt the global cannabis marketplace. This innovative product has strongly addressed the needs and concerns from both the edibles and inhalation sides of the cannabis consumer marketplace. It provides an easy to use, reliable, and healthy alternative dosing format, with the 12/17

13. immediate onset and consistent experience associated with inhalables but without the respiratory health risks, and it eliminates the frustrating delayed effects, imprecision, and frequent overdosing associated with edibles, as well as the calories!” Mr. Kolodinski further stated: “Utilizing PBI’s UST platform, we successfully formulated cannabinoids into a clear, effectively water-soluble, extremely fine nanoemulsion that is conveniently and effectively delivered via an oral mist, providing the consumer with near- immediate onset and rapid peak effects. The consumer testing results announced today herald the dramatic reshaping we expect will follow in the cannabis markets. We are excited to continue to research the impacts of various combinations of cannabinoids, terpenoids, and other compounds to produce highly functional cannaceutical products to improve the lives of people around the world.” Mr. Jeffrey N. Peterson, Chairman of PBI, summarized: “It was extremely satisfying to see our consumer study objectives in assessing THC UltraShear Nanoemulsions for absorption speed and potency, easy and precise dosing, and avoidance of the slow and unreliable under/over-dosing of edibles so well demonstrated. Nonetheless, this was fully anticipated based upon the UltraShear platform’s astonishing demonstrated successes to date across applications spanning industries from pharmaceuticals and nutraceuticals to cosmeceuticals, food and beverages, agrochemicals, and more. PBI’s UltraShear platform offers a game-changing redefinition of the terms of competition for our partners addressing these diverse multi-billion-dollar market opportunities, and we are very excited about the path ahead for PBI and our partners in 2023 and beyond.” About Pressure BioSciences, Inc. Pressure BioSciences, Inc. (OTCQB:PBIO) is a leader in the development and sale of innovative, broadly enabling, pressure-based solutions for the worldwide life sciences and other industries. Our products are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or PCT) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus is in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technologies in the following areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. Forward Looking Statements 13/17

14. This press release contains forward-looking statements. These statements relate to future events or our future financial performance and involve known and unknown risks, uncertainties and other factors that may cause our or our industry’s actual results, levels of activity, performance, or achievements to be materially different from any future results, levels of activity, performance or achievements expressed, implied, or inferred by these forward-looking statements. In some cases, you can identify forward-looking statements by terminology such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “intends,” “anticipates,” “believes,” estimates,” “predicts,” “projects,” “potential” or “continue” or the negative of such terms and other comparable terminology. These statements are only predictions based on our current expectations and projections about future events. You should not place undue reliance on these statements. In evaluating these statements, you should specifically consider various factors. Actual events or results may differ materially. These and other factors may cause our actual results to differ materially from any forward- looking statement. These risks, uncertainties, and other factors include, but are not limited to, the risks and uncertainties discussed under the heading “Risk Factors” in the Company’s Annual Report on Form 10-K for the year ended December 31, 2021, and other reports filed by the Company from time to time with the SEC. The Company undertakes no obligation to update any of the information included in this release, except as otherwise required by law. Pressure BioSciences Gives Guidance for Strong Q4 2022 Sales Followed by Rapid Revenue Growth from Multiple Large Industries in 2023 Foundations Built and Momentum Achieved in 2022 Expected to Drive Company to Significant Revenue Levels, Up-list, and Profitability in 2023: The Stage Has Been Set SOUTH EASTON, MA / ACCESSWIRE / December 22, 2022 / Pressure BioSciences, Inc. (OTCQB:PBIO) (“PBI” or the “Company”), a leader in the development and sale of broadly enabling, pressure-based instruments, consumables, and specialty process development and production services to the worldwide nutraceuticals, cosmetics, biotherapeutics, and food/beverage industries, among others, today offered guidance that the Company believes its Q4 2022 revenue will exceed quarterly revenue for any quarter over the past four years, and may achieve the highest quarterly revenue ever for the Company. PBI believes the foundations built and momentum achieved over the past several months will continue to accelerate, driven primarily by the recent commercial release of its revolutionary Ultra Shear Technology™ (UltraShear™ or UST™) platform. The Company further believes Q1 2023 revenue will greatly exceed Q4 2022’s expected strong results, and that revenues in each successive quarter will surpass the prior quarter’s level. Management expects the Company to reach breakeven/profitability by the end of 2023. Future Outlook (The Stage Has Been Set): Q4 2022 & FY 2023. Revenue Q4 2022: PBI expects to report highest quarterly revenue in past 4 years, likely highest ever. FY 2023: each quarter expected to exceed prior quarter’s revenue. UltraShear revenues expected to top $10M for 2023, beginning with over $1M in Q1 2023. 14/17

15. Agreements (UltraShear Toll Processing) Company ending 2022 with 4 executed agreements; very close to final execution of six or more agreements. Each agreement estimated to yield $1M or more in incremental annual revenue. CBD Expect data releases in Q1 2023 from key opinion leaders in academia and patient treatment/advocacy, supporting PBI’s UltraShear Nano-CBD as among Best-in-Class in Bioavailability globally. THC MA and CA market focus group studies underway to demonstrate expected superior speed of onset and absorption, supporting PBI’s UltraShear Nano-THC as among Best-in-Class in Bioavailability globally. Nutraceutical Supplements Supply agreement with several multi-billion-dollar nutraceutical supplement leaders to be closed by mid-2023; strategic investment expected. Immune Booster Discussions underway with quality-leading, top-selling suppliers seeking assistance to nanosize oil-based active ingredients to be included in new, powerful Immune Booster product…including PBI’s proven capabilities in curcumin, astaxanthin, and CBD. Cosmetics: PBI anticipates the Dr. Denese SkinScience partnership will strongly leverage data generated in 2022 studies of PBI’s UltraShear processed Retinol and Lipid- Soluble Vitamin C, and from current on-going studies, to powerfully validate capabilities of UltraShear-enabled, next-generation, anti-aging serums from Dr. Denese, and will drive new achievements from her existing QVC marketing engine (with over $500M sales over 20 years), starting in Q1 2023. How the Stage was Set: Review of FY 2022 Key Accomplishments April: critical pivot in business focus from PCT Platform to Ultra Shear Technology Platform. April: early access program for UltraShear nanoemulsion processing platform announced. May: first agreement to make UltraShear-based Nano-CBD product – expected 2023 product revenues of $1M. June: PBI partner Ohio State publishes/presents data to support UltraShear role in global $41B plant protein market. July: second $1M+ agreement to process commercial UltraShear-based Nano-CBD products announced. August: third agreement to process commercial Nano-CBD products. Projected FY 2023 revenues to exceed $3M. August: PBI secures pivotal partnership with Dr. Denese SkinScience – QVC leader with $500M 20-year sales. October: PBI and Dr. Denese announce UltraShear-enabled anti-aging and hair regrowth products for 2023 release. 15/17

16. December: Dr. Denese reports data on UltraShear Nano-Retinol greatly exceeds all performance expectations. December: first commercial production of revolutionary UltraShear processed Nano- CBD product shipped and paid. Mr. Richard T. Schumacher, President and CEO of PBI, commented: “Our development of UltraShear over the past 6 years presented a pivotal, game-changing nanoemulsions technology that we believe will restate the basis of competition for a myriad of products across multiple major industries. We have assembled a strong and differentiated IP position and unique engineering and scientific expertise to unleash this opportunity, for PBI and for clients worldwide. We made the critical decision in early 2022 to pivot our business focus and resources almost entirely onto accelerating the UltraShear platform, building commercial-stage UST processing equipment, creating pivotal technology demonstrations with select partners in multiple industries, and accelerating our path to commercial traction and a revenue growth inflection.” Mr. Schumacher concluded: “Our team has navigated a very challenging path to the achievement of this pivot point, and we are proud of what we have built. The stage has been set. Initial partners have been secured and our first commercial UltraShear product has been delivered and paid. Astonishing early demonstrations of UltraShear’s unique differentiation of products continue to mount up, and more partners and early marketing results are gathering already for revenue acceleration in Q1 and throughout all of 2023. We are grateful daily for the patient support of our many long-term investors, and we are excited to have them at our side as PBI enters an exciting new era of revenue growth, profitability, and a planned uplist to a national exchange in 2023.” About Pressure BioSciences, Inc. Pressure BioSciences, Inc. (OTCQB:PBIO) is a leader in the development and sale of innovative, broadly enabling, pressure-based solutions for the worldwide life sciences and other industries. Our products are based on the unique properties of both constant (i.e., static) and alternating (i.e., pressure cycling technology, or PCT) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus is in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally, major new market opportunities have emerged in the use of our pressure-based technologies in the following areas: (1) the use of our recently acquired, patented technology from BaroFold, Inc. (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. 16/17

17. Forward Looking Statements This press release contains forward-looking statements. These statements relate to future events or our future financial performance and involve known and unknown risks, uncertainties and other factors that may cause our or our industry’s actual results, levels of activity, performance, or achievements to be materially different from any future results, levels of activity, performance or achievements expressed, implied, or inferred by these forward-looking statements. In some cases, you can identify forward-looking statements by terminology such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “intends,” “anticipates,” “believes,” estimates,” “predicts,” “projects,” “potential” or “continue” or the negative of such terms and other comparable terminology. These statements are only predictions based on our current expectations and projections about future events. You should not place undue reliance on these statements. In evaluating these statements, you should specifically consider various factors. Actual events or results may differ materially. These and other factors may cause our actual results to differ materially from any forward- looking statement. These risks, uncertainties, and other factors include, but are not limited to, the risks and uncertainties discussed under the heading “Risk Factors” in the Company’s Annual Report on Form 10-K for the year ended December 31, 2021, and other reports filed by the Company from time to time with the SEC. The Company undertakes no obligation to update any of the information included in this release, except as otherwise required by law.Neither PSN nor its owners, members, officers, directors, partners, consultants, nor anyone involved in the publication of this website, is a registered investment adviser or broker-dealer or associated person with a registered investment adviser or broker-dealer and none of the foregoing make any recommendation that the purchase or sale of securities of any company profiled in the PSN website is suitable or advisable for any person or that an investment or transaction in such securities will be profitable. The information contained in the PSN website is not intended to be, and shall not constitute, an offer to sell nor the solicitation of any offer to buy any security. The information presented in the PSN website is provided for informational purposes only and is not to be treated as advice or a recommendation to make any specific investment. Please consult with an independent investment adviser and qualified investment professional before making an investment decision.d. Writer is paid $925/mo by company 17/17