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Bioinspired of Micro-fluidic systems Reach Symposium-2008. Shantanu Bhattacharya Assistant Professor Department of Mechanical Engineering Indian Institute of Technology Kanpur email@example.com Tel: 0512-259-6056. The field of Bio-mimetics.
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Department of Mechanical Engineering
Indian Institute of Technology Kanpur
Alternate Biochip materials
– Poly (dimethylsiloxane) (PDMS)
– Poly (methyl methacrylate) (PMMA)
– Teflon, etc.
• Biological Entities
– Cells, Proteins, DNA
– Frontier of BioMEMS !
Real image of micro-channels
after swelling in solvent
Ref: Sharma et. al., Science, 2007
Relationship with the Biological world
Ref :Stephen D. Centuria, Microsystem Design, Kluwer Academic Publishers, Boston / Dordrecht / London
Lectures, from NanoHUB, Purdue University, West Lafayette, Indiana
Bitensky et.al., 2005, Anal. Chem. 77, 933-937
Beebe et. al., 2000, PNAS, Vol. 97, pp. 13488-13493.
Kim et.al., NSTI-Nanotech 2005, Vol.1
Bhattacharya et. al., JMEMS, 2007.
Chang et. al., Biomedical Microdevices, 2003.
Bhattacharya et. al., Lab chip, 2007, under review.
Ref: Infectious Porcine Diseases, L.R. Sprott and S. Wiske, Agricultural communications, 2002
Sequential fluorescent images of DNA migration behavior in mediums: (a) Nanospehere (b) Agarose and (c) Control Buffer solution without nanosphere 1
 Nanospheres for DNA separation chips
Mari Tabuchi1, 5, 6, Masanori Ueda1, 5, Noritada Kaji1, 5, Yuichi Yamasaki2, 5, Yukio Nagasaki3, 5, Kenichi Yoshikawa4, 5, Kazunori Kataoka2, 5 & Yoshinobu Baba1, 5, 6, 7 , NATURE
DNA Extraction from tissue samples
Imaging of Fluorescence
Glove box for preparing the PCR Mix
Gel electrophoresis of DNA
PCR thermal Cycler
Non fluorescing reference channel for background subtraction
Syringe for injecting PCR mix and sample
Electrodes for Gel electrophoresis
Micro channel filled with agarose gel
Compressed air bottle
Front Elevation View
Reference Solid Core Waveguide for background subtraction
Solid Core Waveguides placed along target DNA regions
Lab-view Operated Solenoid valve
Heaters for PCR
DAQ system hooked to spectrometer will provide the spatial data for the differential intensities
Optical Fibers from Assay
Computer with DAQ card
Pumps in action
Picture of the pumps
Amplified Extract from chip
Amplification performed on .07 pg/ μl sample conc.
Amplified Sample from Conventional M/c
Ref: “Optimization of design and fabrication process for realization of a PDMS-Silicon DNA amplification chip”, by Shantanu Bhattacharya, Venumadhav Korampally, Yuanfang Gao, Maslina Othman, Sheila A. Grant, Steven B. Klieboeker, Keshab Gangopadhyay, Shubhra Gangopadhyay”, Journal of Microelectromechanical systems,Vol.99, pp.1-10, 2007.
2 Basic Capillary Designs
Sample loading sequence in Gel filled channels
1.5% agarose solution in microchannels
300 V for 50 secs
300 V for 25 secs
DNA ladder Trial: 100-1000 bp movement in an Agarose capillary.
Mobility (μ) = 9.101E-4 cm2/ Vsec .
Velocity of the stain=.078 cm/sec
Electric field = 85.7 V/cm
Conventional Electrophoresis Time= 35mins
Requirement : Low voltage capillary electrophoresis system
Ref: Bhattacharya, S., Gangopadhyay K., Gangopadhyay, S., Sharp, P.R., “A low voltage capillary electrophoresis system using platinum doped agarose gels”, (Manuscript to be submitted to Biosensors and bioelectronics).
Platinum nano-particles made in situ
Potassium Chloro-Platinate is reduced by sodium boro-hydride after coating with a monolayer of Mercapto-Succinic acid in a Schlenk line in inert Argon environment. (2 conc. of solution used are 11.6mM and 23.2mM)
Average particle size= 13.16nm,
TEM image of Platinum doped agarose
Back scattered image (FESEM)
Array image of platinum particles embeded in agarose
Ack.: Lou Ross, Randy Tindel and Cheryl Jensen., EMC core
EDS spectra of the Platinized gels
Mobility Enhancement 2 times at 16V/cm
Calculations done using the one dimensional mobility model
µ = v/ E
where , µ = mobility of the stain, v= Velocity (cm/ sec.), E= Electric Field (V/cm)
ZwDielectric Constant Enhancement due to nano-platinum
Electrode width= 17microns
Mobility = ε ε0 ζ / η 
εhas approx. 2 times enhancement
 Rieger T.H., “Electrochemistry”; Prentice Hall, inc., New Jersey, 1987
Ref: Bhattacharya, S., Chanda, N., Grant S.A., Gangopadhyay K., Gangopadhyay, S., Sharp, P.R., “High conductivity agarose nano-platinum composites”, (Manuscript under review in Analytical Chemistry).
Collaborators and Advisors: