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schedule – AIBY 21 Mai 2008

schedule – AIBY 21 Mai 2008. 08:30 complementary competences (groups presentations) proposal of joint projects collaborators/competitors outside of AIBY arguments to EU 12:15 vision AIBY focus (expression systems, products, technologies) next steps (schedule, To Do)

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schedule – AIBY 21 Mai 2008

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  1. schedule– AIBY 21 Mai 2008 • 08:30 • complementary competences (groups presentations) • proposal of joint projects • collaborators/competitors outside of AIBY • arguments to EU 12:15 • vision • AIBY focus (expression systems, products, technologies) • next steps (schedule, To Do) • end 15:00

  2. resources http://www.biotech2008.ch aiby2009 http://cms.biotechlab.net/information/materials_aiby/

  3. Wädenswil Bioprocess TechnologyInstitute of Biotechnology (IBT) School of Life Sciences and Facility Management Zurich University of Applied Sciences (ZHAW) Waedenswil

  4. Zürich University of Applied Sciences the largest multidisciplinary UAS, > 6000 students 8 departments (Wädenswil, Winterthur, Zurich) Masters’ Programme in Pharmaceutical Biotechnology

  5. bioprocess technology high-productivity processes microbial glycosylation(E. coli, P. pastoris) process technology dynamics of physiology fedbatch, flowcytometry process control and simulation (software sensors, predictive control) "software" for biotechnology computer-intensive methods signal extraction, clustering, etc. eLearning www.biotechLAB.net knowledge managementadvanced training

  6. 1 high-productivity processes

  7. methanol:glucose mixtures – trypsinogen

  8. HCD-fedbatch Mut+P. pastoris – trypsinogen open symbols – 100% methanol closed symbols – 40%:60% mixture of glucose and methanol

  9. product forms – Pichia pastoris heat CO2 biomass (CDW) methanol metabolites methanol accumulation proteases product 1 product 2* product 3* ... product i* P. pastoris, Mut+ degradation product a degradation product b degradation product c* ... degradation product j* rP rL O2 OH-/H+ rA * product forms rPproductivity rL rate of proteolytic degradation rA rate of autocatalytic degradation

  10. 2 microbial glycosylation

  11. US$ 5000 per mg – mGM-CSF

  12. yield and glycosylation pattern = f (physiology)

  13. yield and glycosylation pattern = f (physiology) malto-dextrose reference malto-dextrose reference start of induction start of induction end of cultivation end of cultivation Man5GlcNAc2 reference glycans reference glycans

  14. 3 single cell analysis

  15. single cell analysis subpopulations averaged population

  16. vitality by FCM (Mut+P. pastoris)– single cell analysis

  17. complex functionalised biomolecules 1+2 = 4 growth factor(e.g. PDGF ) collagene III (high titre, proper structure) dental implant = +

  18. complex functionalised biomolecules 1+3 = 5 knowledge /advice basehttp://www.biotechLAB.net novel promotors = physiology +

  19. > 1000 proteins – Pichia pastoris biomaterials(polymers) pharmaceuticals enzymes peptides http://faculty.kgi.edu/cregg/Pichia2004.htm

  20. 5 + 1 high-value product categories – yeasts (Pichia pastoris) • therapeutic proteins • (catalytic proteins) • enzymes for the synthesis/modification of pharmaceuticals • new (functional) biomaterials for (e.g. health care) • drug target proteins • proteins for diagnostics

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