Variation in The Expression of TLR3 in Ectopic Pregnancy Saboori Darabi , S 1, 2 ; Zarezadeh , N 1, 2 ; Azadi , R 1, 2 ; Taheri Barayjani , R 1, 2 ; Janan, A 2 ; Lakpour , M 2 ; Khalili , Gh 3 ; Ramazanali , F 2 ; Bidoki , S.K 1 ; Moeini , A 2 ; Aflatoonian, R 2,*
SabooriDarabi, S 1, 2; Zarezadeh, N 1, 2; Azadi, R 1, 2; TaheriBarayjani, R 1, 2; Janan, A 2; Lakpour, M 2; Khalili, Gh3; Ramazanali, F 2; Bidoki, S.K 1;Moeini, A 2; Aflatoonian, R 2,*
1Payame Noor University, Basic Science & Agriculture Unit of Tehran, Tehran, Iran.
2Department of Endocrinology and Female Infertility, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
3Department of Epidemiology and Reproductive Health, Royan Institute for Reproductive Biomedicine, ACECR, Tehran,Iran.
Toll-like receptors (TLRs) form the major family of pattern recognition receptors (PRRs) which are involved in the innate immunity. They recognize pathogen-associated molecular patterns (PAMPs) in bacteria, viruses, fungi and parasites. In human, 10 members of this family have been identified so far. Among these TLRs, TLR3, 7, 8 and 9 designed to recognize nucleic acids and expressed on endosomal membranes. TLR3 recognizes double-stranded RNA (ds-RNA) and is considered to mediate various antiviral responses. Constitutive expression of TLR3 has been reported in female genital tract.
Using RT-PCR the presence of TLR3 gene has been shown in all regions of the FT in both pseudo-pregnant and EP groups. Q-PCR has confirmed relative TLR3 expression in Isthmus, Ampulla and Infundibulum of FTs carrying EP is lower than FTs in pseudo-pregnant women.
Biopsies from Isthmus, Ampulla and Infundibulumof fallopian tube were obtained from women who underwent tubectomy for EP. As control group, human chorionic gonadotropin (hCG) was injected to women who undergoing abdominal hysterectomy for benign gynaecological conditions to produce a state of pseudo-pregnancy. RT-PCR was used to test the existence of TLR3 gene in these sections. Pseudo-pregnant and EP groups were compared with Q-RT PCR for TLR3 gene.
Fig1. TLR3 gene was expressed in all regions of the FTs in both EP and control groups.
It is likely that pregnancy disorders associated with variation in TLR expression. Thus we investigate the role of TLR3 in an Ectopic Pregnancy (EP), a complication of pregnancy in which fertilized egg implants outside the uterus, most often in the Fallopian Tube (FT).
The lower expression of TLR3 in Isthmus, Ampulla and Infundibulum of FTs carrying EP indicates that variation in TLR3 expression can be a risk factor for EP.
Fig2. Results of Semi-quantification for TLR3 gene by using b-actin as a housekeeping gene.
*To whom correspondences should be addressed: R.Aflatoonian@gmail.com