Plant Tissue Culture Application. Development of superior cultivars. Germplasm storage Somaclonal variation Embryo rescue Ovule and ovary cultures Anther and pollen cultures Callus and protoplast culture Protoplasmic fusion In vitro screening Multiplication.
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COMPARISON OF CONVENTIONAL & MICROPROPAGATION OF VIRUS INDEXED REGISTERED RED RASPBERRIES
Plants grow faster than viruses at high temperatures.
Viruses are transported from cell to cell through plasmodesmata and through the vascular tissue. Apical meristem often free of viruses. Trade off between infection and survival.
Adventitious shoots formed from single cells can give virus-free shoots.
Plant from the field
Pre-growth in the greenhouse
35oC / months
Explant INDEXED REGISTERED RED RASPBERRIES→ Callus Embryogenic → Maturation → Germination
Plant germplasm preservation INDEXED REGISTERED RED RASPBERRIES
In vitro Collection INDEXED REGISTERED RED RASPBERRIES
Ways to achieve slow growth INDEXED REGISTERED RED RASPBERRIES
Cryopreservation INDEXED REGISTERED RED RASPBERRIES
Two general types of Somaclonal Variation:
Epigenetic INDEXED REGISTERED RED RASPBERRIES
the study of gene regulation that does not involve making changes to the SEQUENCE of the DNA, but rather to the actual BASES within the nucleotides and to the HISTONES
2n = 2X = 14
2n = 2X = 14
2n = X = 7
H. Bulbosum chromosomes eliminated
“ INDEXED REGISTERED RED RASPBERRIESWide” crossing of wheat and rye requires embryo rescue and chemical treatment to double the number of chromosomes.
Development of hybrid plants through the fusion of somatic protoplasts of two different plant species/varieties
1. isolation of protoplast
2. Fusion of the protoplasts of desired species/varieties
3. Identification and Selection of somatic hybrid cells
4. Culture of the hybrid cells
5. Regeneration of hybrid plants
Isolation of Protoplast INDEXED REGISTERED RED RASPBERRIES
(Separartion of protoplasts from plant tissue)
2. Enzymatic Method
1. Mechanical Method
MicroscopeObservation of cells
Release of protoplasm
Cutting cell wall with knife
Collection of protoplasm
Leaf sterlization, removal of
Protoplast Fusion INDEXED REGISTERED RED RASPBERRIES
(Fusion of protoplasts of two different genomes)
1. Spontaneous Fusion
2. Induced Fusion
Chemofusion- fusion induced by chemicals
NEW SOMATIC HYBRID PLANT FUSION
A procedure that involves retrieval of eggs and sperm from the male and female and placing them together in a laboratory dish to facilitate fertilization
In Vitro Fertilization FUSION