Bacterial Growth and Metabolism -- Chapter 9, & part of Chapter 14 Learn on your own the

1. Bacterial Growth and Metabolism -- Chapter 9, & part of Chapter 14 Learn on your own the materials in the section growth: -- Population growth -- Water and temperature -- Oxygen and acidity -- Other factors (Much of the chapter Self-test focuses on these topics) Also read section in Chap 14 on Microbes and Industry *** You will not be responsible for all of the details of the metabolic pathways covered in this chapter *** Bacterial Growth and Metabolism

2. Bacterial Growth and Metabolism -- Chapter 9 All organisms have three fundamental requirements Carbon Energy Mineral nutrients Bacterial Growth and Metabolism

3. Prokaryotes are much more varied in their sources of C and energy Bacterial autotrophs Phototrophs -- photosynthetic bacteria Chemoautotrophs “Lithotrophs” -- mineral oxidizing bacteria Bacterial Growth and Metabolism

4. Prokaryotes are much more varied in their sources of C and energy Bacterial heterotrophs Photoheterotrophs Chemoheterotrophs saprophytic parasitic Generalists vs fastidious bacteria Bacterial Growth and Metabolism

5. Use of Microbes in Bioremediation Toxic waste sites: PCBs, dioxins, organic solvents, etc Traditional clean up is expensive & inefficient (10’s to 100’s $M) Bioremediation is better alternative Less impact Cheaper Does not merely move the problem Bacterial Growth and Metabolism

6. Cellular Metabolism Why is this topic of importance? Overview of Cellular Respiration identification microbial application chemotherapies What happens to organic molecules that enter a cell? Enzymatically degraded molecular building blocks energy retention Bacterial Growth and Metabolism

7. Glycolysis Converts sugars into Pyruvate Requires energy investment Energy yield ATP NADH Glycolysis Bacterial Growth and Metabolism

8. Aerobic Cellular Respiration Transition reaction Krebs cycle Electron transport Energy yield ATP NADH e- Transport Bacterial Growth and Metabolism

9. Anaerobic metabolism Bacterial O2 requirements Obligate aerobes Facultative Microaerophiles Obligate anaerobes Fermentation Functions End products Alternative terminal e- acceptors e.g., CO2, SO4 Bacterial Growth and Metabolism

10. Industrial Uses of Bacteria -- chapter 14 Other fermentation products Antibiotics and pharmaceutics Figure 5.12 Bacterial Growth and Metabolism

11. Beer production Normal beer: only yeast … boring! Lambic beer … yeast and bacteria! Yeast CO2 production Bacterial Growth and Metabolism

12. Cheese production Involves bacteria (and sometimes fungi) Curdling stage Ripening stage Bacterial Growth and Metabolism

13. Autotrophic bacteria CO2 is carbon source Energy from light (phototrophs) or inorganics (chemoautotrophs) Phototrophs Purple sulfur bacteria -- anoxygenic Ps Cyanobacteria -- oxygenic Ps Bacterial Growth and Metabolism

14. Cyanobacteria “blue-green” pigmentation Habitat Stromatolites Visit NASA’a “Stromatolite Explorer” on-line Oxygenic Photosynthesis Bacterial Growth and Metabolism

15. Purple sulfur bacteria Photosynthesis Habitat Pigmentation Sulfur granules Anoxygenic Photosynthesis Bacterial Growth and Metabolism