BIOT 2922. Tissue Culture Methods. Objectives. Work aseptically. Maintain mammalian cells in healthy, uncontaminated state for 2-4 weeks. Manipulate mammalian cells. Cell lines. Chinese hamster ovary (CHO) cells Introduced in the 1960s. Used in a cultured monolayer in culture flasks.
Tissue Culture Methods
Confluency: coverage or proliferation that the cells are allowed over or throughout the culture medium