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Validation of DNA Testing for Carcass Traits

Validation of DNA Testing for Carcass Traits. John Pollak Cornell University. Objectives of DNA Project Team. Develop protocol for validation (or not) of results with independent (field) data. DNA team: Richard Quaas (CU), Rohan Fernando (ISU) and Mark Thallman (MARC).

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Validation of DNA Testing for Carcass Traits

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  1. Validation of DNA Testing for Carcass Traits John Pollak Cornell University

  2. Objectives of DNA Project Team • Develop protocol for validation (or not) of results with independent (field) data DNA team: Richard Quaas (CU), Rohan Fernando (ISU) and Mark Thallman (MARC)

  3. DNA Tests for Carcass Merit Traits • GeneStar Marbling • Mu-Calpain • Calpistatin • Leptin • Marked QTL from NCBA Carcass Merit Project (genes unknown)

  4. NBCEC Data • Marbling scores on 277 cattle w/ genotypes • Progeny of 19 sires: +/- & +/+ • genotyped Simmental bulls • ASA progeny test data (mostly CMP) • 11 contemporary groups • Marbling scores on 272 contemporaries • 49 total sires of progeny w/ MS

  5. Slide courtesy of Jay Hetzel 0 Zero copies One copy Two copies A simple result

  6. NBCEC vs. Genetic SolutionsMarbling Score Results

  7. NBCEC vs Genetic Solutions% Choice Results

  8. mu-Calpain • mu-Calpain or Calpain 1 • Post-mortem degradation of myofibrillar proteins • Tenderness • W-B Shear force • Calpastatin (GeneStar tenderness) • regulates Calpain

  9. NBCEC mu-Calpain Analysis 2 SNP markers in calpain gene • Analyzed W-B shear force • Each marker separately • Together • Additive • Interaction

  10. Mu-Calpain: 2 Additive Genotypes

  11. Mu-Calpain: 2 Additive Genotypes

  12. Other’s? Leptin Meriel In our population of cattle used for validation we did not find an effect of the polymorphism in the Leptin gene for marbling.

  13. NCBA Carcass Merit Project Objectives: • Investigate genetic components for tenderness and sensory panel information including potential for developing EPD’s and QTL for DNA testing.

  14. DNA Component of CMP Validation of QTL discovery Texas A&M Angelton Project (Angus and Brahman cross cattle) 11 QTL candidates selected 12 phenotypes analyzed

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