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Explore the impact of collagen concentration on cell growth using Luminescence (R.L.U.) measurement. Cells were cultured in 96-well dishes at varying densities and collagen concentrations over 7 days. CellTiter-Glo assay was used to measure cell viability. The study indicates the optimal collagen concentration for cell growth.
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Supplemental Figure 4 a 1000, 2000, 4000, 8000 cells/well into 96 well dish 10, 20, 40, 80 cells/well into 96 well dish R.L.U. measurement R.L.U. measurement CellTiter-Glo CellTiter-Glo 10% FBS DMEM 10% FBS DMEM 7 days 6 hr collagen concentration Collagen 1 gel (2.4, 1.2 mg/ml) Collagen 1 gel (2.4 mg/ml) Collagen 1 gel (2.4 mg/ml) Luminescence (R.L.U.) (mg/ml) (cell number/well) b (cell number/well) Luminescence (R.L.U.)