separating ferrocene and acetylferrocene by adsorption column chromatography n.
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Separating Ferrocene and Acetylferrocene by adsorption column chromatography - PowerPoint PPT Presentation


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Separating Ferrocene and Acetylferrocene by adsorption column chromatography. Acetylferrocene. Ferrocene. Adsorption column chromatography. In Column chromatography, the stationary phase (absorbent) is packed in a glass column The mobile phase (solvent), moves through the packed column

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adsorption column chromatography
Adsorption column chromatography
  • In Column chromatography, the stationary phase (absorbent) is packed in a glass column
  • The mobile phase (solvent), moves through the packed column
  • The solvent that is used as the mobile phase is called an eluent
atracttion
Atracttion
  • A mixture of compounds is added to the eluent
  • As the eluent moves through the column the mobile phase and stationary phase interact with the compounds.
  • If a compound is more attracted to the mobile phase it will move rapidly through the column and Elute, (leave)
  • If a compound is more attracted to the stationary phase it will move slower.
atracttion1
Atracttion
  • The more polar compounds in the mixture will bind strongly to the absorbent : less polar compounds not so strong.
  • Separtion occurs when an eluent with a low to moderate polarity is passed through the column.
  • The less polar compounds in the mixture will move throught the column
  • The strongly polar compounds will remain with the absorbent.
polarity of eluents
Polarity of eluents
  • In some cases two eluents maybe used, the eluent with the lowest polarity is used first
slide8

Ferrocene = Yellow

Acetylferrocene = orange