Chromatography Column selectivity - a. Elution volume: V m =mobile phase interstitial volume V s =stationary phase volume V R is the retention volume of an unretained compound + an additional volume of mobile phase required to elute a compound
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Column selectivity - a
column column capacity
efficiency selectivity factor
Time required for a given analysis
Plot of tR and R versus k’
The effect of isotherm shape on peak shape and retention time as a function of sample size