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Osmosis Experiment Protocol: Observing Potato Tubers in Different Sucrose Solutions

Conduct an osmosis experiment using potato tubers and various sucrose solutions to learn about hypertonic, isotonic, and hypotonic solutions. Observe changes in potato texture to understand water potential and turgor pressure. Apply knowledge to match potato tubers with solution concentrations.

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Osmosis Experiment Protocol: Observing Potato Tubers in Different Sucrose Solutions

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  1. Agenda12/6—Day 5 • Get out your lab books • Obtain a potato tuber • Put the tubers in order of how you think osmosis occurred based on the concentrations

  2. Procedure • Get a paper towel • Label the with the 6 colors of the solutions • As you remove the potato, place it under the appropriate color • Handle the potato . What does it feel like? • Can you use your knowledge to explain what is happening in each potato?

  3. Solutions • Distilled water=Blue • .2 M Sucrose=Green • .4 M Sucrose=Red • .6 M Sucrose=Yellow • .8 M Sucrose=Orange • 1.0 M Sucrose=Clear

  4. Collins • Explain what knowledge you used to match the potato tuber to it’s respective sucrose solution • Hypertonic • Isotonic • Hypotonic • Water potential • Turgid • Plasmolysis • flaccid

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