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Impact of MetD Operon Overexpression on L- and D-Methionine Uptake in Bacterial Mutants

This study investigates the effects of overexpressing the metD operon on the uptake of 1-[14C]L-methionine and 1-[14C]D-methionine in various bacterial strains, including wild type and mutants Δabc-yaeE and ΔyaeC. Bacteria were cultivated in M9 minimal medium supplemented with ampicillin and arabinose. The uptake is measured in nmol/mg dry weight over a specified time period, providing insights into the metabolic pathways involved and demonstrating the differences in methionine uptake among the different strains with and without the metD operon.

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Impact of MetD Operon Overexpression on L- and D-Methionine Uptake in Bacterial Mutants

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  1. 6 4 L-methionine uptake (nmol/mg dry weight) 2 0 3 2 D-methionine uptake (nmol/mg dry weight) 1 0 8 6 2 10 0 4 Time (min) Figure S2. Effects of overexpression of the metD operon on uptake of 1-[14C]L- methionine (A) and 1-[14C]D-methionine (B) by the wild type, the abc-yaeE and the yaeC mutant. Bacteria were grown in M9 minimal medium plus ampicillin and arabinose at 100 g/ml and 2 mM, respectively. Uptake was performed as described in Figure 2. , wild type BW25113 (pBAD24); , wild Type BW25113 (pBAD24-metD); , abc-yaeE (pBAD24); , abc-yaeE (pBAD24-metD); , yaeC (pBAD24); and ,yaeC (pBAD24-metD).

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