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6. 4. L-methionine uptake (nmol/mg dry weight). 2. 0. 3. 2. D-methionine uptake (nmol/mg dry weight). 1. 0. 8. 6. 2. 10. 0. 4. Time (min). Figure S2. Effects of overexpression of the metD operon on uptake of 1-[ 14 C]L-

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  1. 6 4 L-methionine uptake (nmol/mg dry weight) 2 0 3 2 D-methionine uptake (nmol/mg dry weight) 1 0 8 6 2 10 0 4 Time (min) Figure S2. Effects of overexpression of the metD operon on uptake of 1-[14C]L- methionine (A) and 1-[14C]D-methionine (B) by the wild type, the abc-yaeE and the yaeC mutant. Bacteria were grown in M9 minimal medium plus ampicillin and arabinose at 100 g/ml and 2 mM, respectively. Uptake was performed as described in Figure 2. , wild type BW25113 (pBAD24); , wild Type BW25113 (pBAD24-metD); , abc-yaeE (pBAD24); , abc-yaeE (pBAD24-metD); , yaeC (pBAD24); and ,yaeC (pBAD24-metD).

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