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Figure 1. 50 ng/ml NGF. 5 ng/ml NGF. A. NGF + SB203580. Control. NGF + SB202190. NGF. B. 8. 6. p38 MAPK phosphorylation (Fold stimulation). 4. 2. 0. 10. 20. 30. Time (min). P-p38 T-p38. 5 ng/ml NGF. P-p38 T-p38. 50 ng/ml NGF. Time (min). 0. 1. 3. 5. 10. 15.

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  1. Figure 1 50 ng/ml NGF 5 ng/ml NGF A NGF + SB203580 Control NGF + SB202190 NGF B 8 6 p38 MAPK phosphorylation (Fold stimulation) 4 2 0 10 20 30 Time (min) P-p38 T-p38 5 ng/ml NGF P-p38 T-p38 50 ng/ml NGF Time (min) 0 1 3 5 10 15 20 30 C 8 6 p38 MAPK phosphorylation (Fold stimulation) 4 2 0 1 10 100 1000 Log [NGF] (ng/ml) P-p38 T-p38 0 5 10 30 50 100 300 1 NGF (ng/ml)

  2. Figure 2 Control 5 ng/ml NGF 50 ng/ml NGF P-p38 T-p38 NGF PTX Basal 5 ng/ml NGF 6 PTX * * * * 4 * * 2 0 P-p38 T-p38 p38 MAPK phosphorylation (Fold stimulation) 6 PTX * * * 4 # # # 2 0 P-p38 T-p38 Gz Gi2 GoA Gi2/z GoACI Control A 8 * * 6 # p38 MAPK phosphorylation (Fold stimulation) # 4 2 0 P-p38 T-p38 PTX B C

  3. Figure 3 A IP IP Lysate Lysate TrkA TrkA IgG IgG WB P-TrkA Gi2 Gz Go NGF NGF B 6 * PTX 4 2 0 P-p38 T-p38 p38 MAPK phosphorylation (Fold stimulation) 6 4 # 2 0 P-p38 T-p38 PTX Gi2 Gi2GT

  4. Figure 4 Basal 5 ng/ml NGF 5 ng/ml NGF + inhibitor A B * 14 * 6 ERK JNK * * 12 * * MAPK phosphorylation (Fold stimulation) p38 MAPK phosphorylation (Fold stimulation) 4 10 8 # 2 # # 4 0 0 P-MAPK T-MAPK P-p38 T-p38 NGF NGF NGF U0126 U0124 U0124 U0126 Control Control Control SP600125 SP600125

  5. Figure 5 A B Basal 5 ng/ml NGF 5 ng/ml NGF + inhibitor 6 * * * * * * * * * * * 4 p38 MAPK phosphorylation (Fold stimulation) # # 2 # 0 P-p38 T-p38 W7 PP1 PP2 PP3 NGF NGF KN62 AG490 U73122 U73343 SU6656 Control Control AG1295 Calphostin C Staurosporine C 3 * 2 # Src phosphorylation (Fold stimulation) 1 0 P-Src T-Src PTX

  6. Figure 6 NGF Trk A receptor GIPC1 GAIP Gi2 Src G Src Gi2 Crk Rho GEF C3G Ras Rac/ cdc42 Rap1 RhoA B-Raf MEKK1-4 MLKs MEK1/2 MKK4 MKK3/6 p38 MAPK

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