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This study investigates how PAP1 and HY5 affect hypocotyl length and gene expression in Arabidopsis, using dark, far-red, and white light conditions. Results show a correlation between PAP1/HY5 levels and hypocotyl growth.
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Sup Fig. 1. pap1hy5-1 pap1hy5-2 Col-0 pap1 No-0 hy5 pap1hy5-1 pap1hy5-2 A Col-0 pap1 No-0 hy5 PAP1 B Actin2 Hypocotyl length (mm) C Dark Far-red White
Sup Fig 2. A pap1Dhy5-7 pap1Dhy5-4 pap1Dhy5-8 pap1D Col-0 hy5 B PAP1 Col-0 pap1D hy5 pap1Dhy5-4 pap1Dhy5-7 pap1Dhy5-7 pap1Dhy5-7 pap1Dhy5-7 pap1Dhy5-7 pap1Dhy5-4 pap1Dhy5-4 pap1Dhy5-4 pap1Dhy5-4 pap1Dhy5-8 pap1Dhy5-8 pap1Dhy5-8 pap1Dhy5-8 pap1D pap1D pap1D pap1D Bar/PAP1 Col-0 Col-0 Col-0 Col-0 hy5 hy5 hy5 hy5 pap1Dhy5-4 pap1Dhy5-7 pap1Dhy5-8 pap1D Col-0 hy5 C Dark Red Far-red Blue Dark Red Far-red Blue PAP1 18S RNA Hypocotyl length (mm) D E
Sup Fig 3. Enrichment (% IP/Input) rDNA A C E CHS-D CHI-C PAP1
Sup Fig 4. GUS activity (nmole 4MU/min/mg) PAP1 –P1::GUS Mock
Sup Fig 5. G/ACE box PCE box -872 ~ -863 -186 ~ -177 ATG UF3GT -1164 ~ -1159 -252 ~ -161 ATG DFR ATG -182 ~ -177 -868 ~ -863 -452 ~ -443 LDOX -452 ~ -447 200 bp