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Quantifying Purified GST-Nups: Titration Study of Abundance with Various Proteins

This study involves titration of different GST-Nup proteins including GST-Nsp1, GST-Nup116, GST-Nup100, GST-Nup145, GST-Nup1, GST-Nsp1, and GST-Nup42 to determine their abundance levels, aided by BSA standards and varying IPTG concentrations.

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Quantifying Purified GST-Nups: Titration Study of Abundance with Various Proteins

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  1. Titration of Abundance for Purified GST-Nups 1 MW 2 -IPTG 3 +IPTG 4 MSS 5 blank 6 5 mL GST-Nup 750 mL GST-Nup 8 1mg BSA 9 2mg BSA 10 4mg BSA 1 MW 2 -IPTG 3 +IPTG 4 MSS 5 blank 6 5 mL GST-Nup 750 mL GST-Nup 8 1mg BSA 9 2mg BSA 10 4mg BSA 1 MW 2 -IPTG 3 +IPTG 4 MSS 5 blank 6 5 mL GST-Nup 750 mL GST-Nup 8 1mg BSA 9 2mg BSA 10 4mg BSA A GST-Nsp1h (1-172) B GST-Nup116h (165-715) C GST-Nup100h (1-640) D GST-Nup145h (1-242) E GST-Nup1h (798-1076) F GST-Nsp1 (377-471) K>R H GST-Nup42 (1-382) G GST-Nsp1 (377-471) wild type

  2. Titration of Abundance for Purified GST-Nups

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