cell structure and function
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CELL STRUCTURE AND FUNCTION. Lab 3. Objectives. Be able to make a wet mount of any cellular material provided and focus on a cell. Be able to stain cells with either Lugol’s solution or methylene blue. Be able to determine the length and width of cells in micrometers and/or millimeters.

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objectives
Objectives
  • Be able to make a wet mount of any cellular material provided and focus on a cell.
  • Be able to stain cells with either Lugol’s solution or methylene blue.
  • Be able to determine the length and width of cells in micrometers and/or millimeters.
  • Be able to diagram any cell observed through the microscope.
  • Be able to locate the following structures in an onion cell:
  • cell wall
  • vacuole
  • nucleus
  • cytoplasm
  • cell membrane.
the cell
THE CELL
  • The cell concept is basic to understanding the activities and characteristics of organisms.
  • Cells are the units of structure and function of an organism.
  • As units, they reflect the abilities of the organism as a whole. Cells are of interest because of their variety and also because of their similarities.
slide4
In this exercise, we will examine the cell. We will try to determine the three-dimensional shape of theses cells and identify some of their structures.
  • The cell is compose by three principal compartiment:

1.- Plasmatic membrane

2.- Cytoplasm (organelles)

3.- Nucleous

slide5
Plasma Membrane

Figure 3.3

slide6
Cytoplasma
  • It is the specialized living material of cells
  • It lies between the plasma membrane and the nucleus
  • Numerous small structure (organelles) are part of the cytoplasma, along with the fluid that serves as the interior

environment of each cell

slide7
Organelles
  • Ribosomes
  • Endoplasmic reticulum
  • Golgi apparatus
  • Mitocondria
  • Lysosomes
  • Centrioles
slide9
Nucleus
  • Contains nuclear envelope, nucleoli, chromatin, and distinct compartments rich in specific protein sets
  • Gene-containing control center of the cell
  • Contains the genetic library with blueprints for nearly all cellular proteins
  • Dictates the kinds and amounts of proteins to be synthesized
slide10
Nucleoli
  • Dark-staining spherical bodies within the nucleus
  • Site of ribosome production
slide11
Nucleus

Figure 3.28a

slide15
During this lab exercise you can:
  • Prepare sections of onion membrane, make a temporary wet mount, and view the specimen through a microscope.
  • Prepare sections of onion membrane, make a stained temporary wet mount, view through a microscope, and sketch a generalized cell.
  • Collect cheek epithelial cells, make a stained temporary wet mount, view through a microscope, and make drawing of the structure of a generalized cheek cell.
  • Make a temporary wet mount of Elodea, view it through a microscope and make drawing of the structure of a generalized cheek cell.
slide16
Locate the following structures:
  • cell wall (thick, clear band which surrounds the cell)
  • cytoplasm (granular material in the cell next to the cell wall)
  • nucleus (football-shaped object in the cell)
  • vacuole (large, central, nongranular space)
  • cell membrane (thin membrane just inside the cell wall. If you place a drop of 5% salt solution on the onion tissue, the size of the cell will shrink and the cell membrane will pull away from the cell wall revealing the cell wall and the cell membrane are separate structures).
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