1 / 13

Principles of genetic engineering

Principles of genetic engineering. L Mathias. What is genetic engineering. Genetic engineering, also known as recombinant DNA technology , means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype.

Download Presentation

Principles of genetic engineering

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Principles of genetic engineering L Mathias

  2. What is genetic engineering • Genetic engineering, also known as recombinant DNA technology, means altering the genes in a living organism to produce a Genetically Modified Organism (GMO) with a new genotype. • Various kinds of genetic modification are possible: inserting a foreign gene from one species into another, forming a transgenic organism; altering an existing gene so that its product is changed; or changing gene expression so that it is translated more often or not at all.

  3. Basic steps in genetic engineering • Isolate the gene • Insert it in a host using a vector • Produce as many copies of the host as possible • Separate and purify the product of the gene

  4. Step 1: Isolating the gene

  5. Step 1: Alternative method (using reverse transcriptase) • Reverse transcriptase • mRNA converted into cDNA • Complementary strand produced using DNA polymerase • Advantage – more mRNA in cell than DNA

  6. Step 2: Inserting gene into vector • Vector – molecule of DNA which is used to carry a foreign gene into a host cell

  7. Step 3: inserting vector into host • See worksheet

  8. Replica plating

  9. Step 4: Multiplication of the host cells by cloning • Large scale fermenters by cloning • All genetically identical because of asexual reproduction

  10. Step 5: Extraction of desired gene product

  11. This powerpoint was kindly donated to www.worldofteaching.com http://www.worldofteaching.com is home to over a thousand powerpoints submitted by teachers. This is a completely free site and requires no registration. Please visit and I hope it will help in your teaching.

More Related