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Termite Feeding and the Protozoa

Termite Feeding and the Protozoa. +. =. Spirotrichonympha leidyi Koidzumi. Holomastigotoides hartmanni Koidzumi. Formosan Subterranean Termites (Kingdom Animalia). Pseudotrichonympha grassii Koidzumi. Protozoa (Kingdom Protista). Symbiotic Relationship.

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Termite Feeding and the Protozoa

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  1. Termite Feeding and the Protozoa + =

  2. Spirotrichonympha leidyi Koidzumi Holomastigotoides hartmanni Koidzumi Formosan Subterranean Termites (Kingdom Animalia) Pseudotrichonympha grassii Koidzumi Protozoa (Kingdom Protista) Symbiotic Relationship Association of two dissimilar organisms in a beneficial relationship +

  3. The Protozoan receives: • A home (shelter) by living in the termite gut. • Cellulose (food)from wood that the termite eats • The cellulose is digested and acetate, carbon dioxide, hydrogen, and methane are produced and released. • The Termites receives/absorbs: • Acetate produced by the Protozoa. • The termites use the acetate as • their energy source to carry out daily bodily • functions. The Benefits of the Symbiotic Relationship

  4. The Transfer of Protozoa in a NEW colony The queen and the king are responsible for feeding and passing on the protozoa to the offspring in the new colony.

  5. The Transfer of Protozoa in an OLDER Colony The workers are responsible for feeding and transferring the protozoa in an older colony. • Stomadeal Feeding • Passing partially digested food with the mouth to another termite. • Proctodeal Feeding • Receiving food from the anus.

  6. head thorax abdomen External Anatomy of a TermiteIdentification of Body Sections 1.Head = The eyes, antennae, and mouthparts are on the head. 2.Thorax = The 3 pairs of legs are attached to the thorax. 3.Abdomen = The abdomen holds all reproductive organs.

  7. 5. Digested material passed out of the anus to feed others or build carton Internal Anatomy of a TermiteHow wood is digested 1. Pieces of wood enters the mouth. 2. Wood is ground in the crop. 3. Wood particles enters the hind gut and are consumed by the protozoans, who release acetate, carbon dioxide, hydrogen, and methane. 4. Acetate release by protozoa is absorbed by the termite

  8. Omnivore Energy Source Carnivore Consumers/Hetertrophs Producers Herbivore SOIL BACTERIA (decomposers) Carnivore Producer

  9. Extracting Protozoa from Termite Gut: Materials • 5-6 Formosan Subterranean Termites (workers) • 2 Forceps • 1 Light microscope • 1Microscope slide • 1 Cover slip • 2 drops of saline solution • 1 disposable pipette • Kimwipes

  10. Microscope slide with a drop of saline solution Extracting Protozoa from Termite Gut: Method/Procedure • Place a drop of saline solution on a microscope slide.

  11. head thorax abdomen Extracting Protozoa from Termite Gut: Method/Procedure 2. Gently grab the termite’s head and thorax with a forcep.

  12. head thorax abdomen Extracting Protozoa from Termite Gut: Method/Procedure 3. Locate the tip of the abdomen and the end of the termite’s gut. 4. With the second forcep, grab and pull out the termite’s gut.

  13. Cover slip Microscope slide with a drop of saline solution and termite intestine Extracting Protozoa from Termite Gut: Method/Procedure 5. Place the gut in the saline solution on the microscope slide. 6. Slowly lower a cover slip on to the sample and press down gently.

  14. Microscope slide with a drop of saline solution and termite gut Extracting Protozoa from Termite Gut: Method/Procedure 7. If there is extra saline solution on the microscope slide, use a paper towel to wipe the slide clean.

  15. My laboratory Worksheet Extracting Protozoa from Termite Gut: Method/Procedure 8. Observe the gut sample under the microscope and complete the laboratory worksheets.

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