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LacZ. wtALK1/HA. - TGF- b. - TGF- b. - TGF- b. +TGF- b. +TGF- b. +TGF- b. MEEC BRE-luc. 120. 100. 80. Luciferase activity (arbitrary unit s ). 60. 40. 20. 0. -. -. ALK1- RNAi. BAEC. A. MEEC BRE-luc. B. 45 min. 45 min. 90 min. 90 min. 180. TGF- b:. 3h. 2h. 2h. 3h.

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  1. LacZ wtALK1/HA -TGF-b -TGF-b -TGF-b +TGF-b +TGF-b +TGF-b MEEC BRE-luc 120 100 80 Luciferase activity (arbitrary units) 60 40 20 0 - - ALK1- RNAi BAEC A. MEEC BRE-luc B. 45 min 45 min 90 min 90 min 180 TGF-b: 3h 2h 2h 3h 4h 4h 0 0 160 Phospho- Smad1/5 140 120 Phospho- Smad2 100 Luciferase activity (arbitrary units) 80 60 HA 40 20 actin 0 - ALK1 Smad5 ALK1 + Smad5 C. D. MEEC BRE-luc 80 70 60 50 Luciferase activity (arbitrary units) 40 30 20 10 ALK1 RNAi + hALK1 0 - siRNA- Smad5 Smad5 RNAi + mSmad5

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