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Investigating Significant Mutations: Case Studies in MCL1 and ZYX Proteins

Explore the implications of MCL1 K123A mutation in cancer and delve into the biology of novel protein ZYX, focusing on its binding to mTOR, similarity to bTrCP, deletion in cancers, and phosphorylation site. Learn about experimental approaches to understand these proteins.

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Investigating Significant Mutations: Case Studies in MCL1 and ZYX Proteins

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  1. Question 1: • You have joined a lab for your graduate studies and your project is to study a mutation in MCL1 that was discovered in an Argentinian family with a history of cancer. • This mutation is K123A. From patient biopsies, your initial observation is that MCL1 is localized to mitochondria in tumor cells and nucleus in the normal cells of a cancer patient. • Why might this observation be significant? Describe several experiments you could undertake to understand the biology of K123A mutation. • Question 2: • You have finished your PhD and you have just started your post-doc training. Your project is to investigate a novel protein ZYX with the following characteristics and observations: • 1. ZYX binds to the kinase mTOR • 2. ZYX has 89% similarity to the E3 ligase bTrCP. • 3. ZYX is deleted in 3% of human cancers • 4. ZYX has a PKB/AKT consensus phosphorylation site as follows: WRMRAGTVP. • Describe various experiments to explore the biology of your novel protein ZYX.

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