1 / 73

Introduction to next -generation sequencing technologies and bioinformatics

Introduction to next -generation sequencing technologies and bioinformatics. Nicholas D. Socci Bioinformatics Core Memorial Sloan Kettering Cancer Center. Part i. Disclosure. Disclosure (being honest). Bioinformatics/ Computational Biology. Disclosure (being honest). Bioinformatics/

sgarrido
Download Presentation

Introduction to next -generation sequencing technologies and bioinformatics

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Introduction to next-generation sequencing technologies and bioinformatics Nicholas D. Socci Bioinformatics Core Memorial Sloan Kettering Cancer Center

  2. Part i Disclosure

  3. Disclosure(being honest) Bioinformatics/ Computational Biology

  4. Disclosure(being honest) Bioinformatics/ Computational Biology Next Generation Sequencing Bioinformatics/Computational Biology

  5. Disclosure(being honest) Bioinformatics/ Computational Biology Next Generation Sequencing Bioinformatics/Computational Biology

  6. Disclosure(being honest) Subject area covered in this talk Bioinformatics/ Computational Biology Next Generation Sequencing Bioinformatics/Computational Biology

  7. Useful fact #1Understand the data • Because Bioinformatics draws on many disciplines: • Biology • Mathematics/Statistics • Computer Science It can be hard to understand all parts of a project/problem But you have to, either individually or in collaboration.

  8. BIOinformatics • For the computer scientist, mathematics, statisticians, physicists: • Need to learn some biology, both: • General: DNA vs RNA • Specific to problem working • No time for me to talk about it hear though

  9. Part ii Sequencing Technologies (lite)

  10. Part of understanding the data • Is understanding how it was measured

  11. DNA sequencing “history” Efficiency (bp/person year) 1870 MIESCHER : Discovered DNA 1 1940 AVERY: proposed DNA as “genetic material” 15 WATSON & CRICK: double-helix structure 1953 150 1,500 1977 SANGER/Dideoxy Termination MAXAM & Gilbert/ Chemical Degradation 15,000 1980 25,000 PCR sequencing concept was introduced 1986 PARTIAL AUTOMATION 50kb to 100kb Human genome project 1995 FULL AUTOMATION 120MB/person week 2005 NEXT GENERATION SEQUENCING 60GB week 2009 NEXT NEXT GENERATION SEQUENCING

  12. Sanger sequencing 1- Labeling DNA fragments are labeled by using fluorescently labeled ddNTP 2- Capillary electrophoresis 3- Reading

  13. Sanger sequencing • Two main problems: • Not very high throughput • Expensive • Example: Human genome project • 13 years • $2.7 billions • Impossible to use if one thinks about sequencing patients genome for example • Need for new sequencing technologies to reach the $1000 genome goal. • NEXT GENERATION SEQUENCING INSTRUMENTS

  14. Major NextGen Technologies • Sequencing by ligation • SOLiD (ABI/Lifetech); dibaseprobes double reads • some test most accurate • Short (75x35) medium throughput • Sequencing by synthesis • 454 Roche – pyrosequencing • Homopolymer issue, very expensive • Long reads ~ 400 • Ion Torrent/Proton (LifeTect) use pH instead of fluorophores • homopolymers, expense could go down • medium reads ~ 150 • Throughput could scale rapidly • IlluminaHiSeq/MiSeq • Seems to be the best trade off of accuracy; throughput; cost and length

  15. Different platforms but same concept DNA Fragmented DNA “sequencing library” Ligation of Adaptors PCR cluster for Illumina Clonal amplification of the different fragments Emulsion PCR for 454, SOLiD, PGM Sequencing flavors -sequencing by synthesis -Pyrosequencing -sequencing by ligation -sequencing by measuring pH changes Sequencing

  16. Cluster Generation by Bridge Amplification In contrast to the 454 and ABI methods which use a bead-based emulsion PCR to generate "polonies", Illumina utilizes a unique "bridged" amplification reaction that occurs on the surface of the flow cell. The flow cell surface is coated with single stranded oligonucleotides that correspond to the sequences of the adapters ligated during the sample preparation stage. Single-stranded, adapter-ligated fragments are bound to the surface of the flow cell exposed to reagents for polyermase-based extension. Priming occurs as the free/distal end of a ligated fragment "bridges" to a complementary oligo on the surface. Repeated denaturation and extension results in localized amplification of single molecules in millions of unique locations across the flow cell surface. This process occurs in what is referred to as Illumina's "cluster station", an automated flow cell processor. http://seqanswers.com/forums/showthread.php?t=21

  17. Sequencing by Synthesis A flow cell containing millions of unique clusters is now loaded into the 1G sequencer for automated cycles of extension and imaging. The first cycle of sequencing consists first of the incorporation of a single fluorescent nucleotide, followed by high resolution imaging of the entire flow cell. These images represent the data collected for the first base. Any signal above background identifies the physical location of a cluster (or polony), and the fluorescent emission identifies which of the four bases was incorporated at that position. This cycle is repeated, one base at a time, generating a series of images each representing a single base extension at a specific cluster. Base calls are derived with an algorithm that identifies the emission color over time. At this time reports of useful Illumina reads range from 26-50 bases.

  18. Pac-Bio (next-next gen) Long reads!

  19. Part iii Algorithms/Pipelines

  20. Data Sizes per Analysis Phases Raw Data (Images) Terabytes/Run 1o anal 2o analysis 3o anal BCLs 250Gb tmp SNP Tbl Exprs Val Profiles Reads (+Quals) Dedicated Cluster on Instrument Map Files BAMs (1-100Gb) Biology Computer Science

  21. Next Generation Resequencing Steps Secondary Analysis Tertiary Analysis SNP variant runs: Calling: Unified Genotyper, Haplotype caller. Annotation: coding/non-coding, syn/non-synonymous, Functional (HUGE) Structural Copy number rearrangments RNA-seq: Expression matrix: Genes, Transcripts, Exons Splicing ?Fusions? ChIP-seq/Methylation: MACS, Custom • Mapping: to known genome or reference database reads. • DNA mappers • BWA, SHRiMP, Bowtie • RNA mappers (spliced): • TopHat, rnaStar • BAM Processing: • MarkDups • Indel/Realign • BaseQ Recalibration • QC Reports: • New BAM Compression

  22. RNAseq programs/ workflows Alamancos, et. al. http://arxiv.org/abs/1304.5952

  23. Two very important points • Impossible to get a comprehensive list of algorithms/programs • Too many • Constantly changing • Updateing • New ones added/old ones go away • Huge job staying current, do your own research • However should try to stick to standard data formats: • FASTQ • SAM/BAM • VCF • Resist temptation to invent your own

  24. Missing huge area • de novo, looking for new or novel sequences: What most think sequencing is • Sequencing the human genome • Lots of work on sequencing new organisms • Not my specialty (1 yeast project; will last a lifetime) • Some “de novo” stuff in resequencing: • Splicing, fusions, structural rearrangements • But most resequencing is either • Small changes (variations) to the reference • Counting: RNAseq, ChIPseq, X-seq

  25. mini de novo: looking for novel exons • Not new sequence but new “structure” • Androgen Receptor in Prostate Cancer Cell-line

  26. Focus on one pipeline • Detection of variants • SNV or small Insertions/Deletions • My primary work is in Somatic Variants (differential) • Tumor vs Normal • Metastasis vs Primary • However most of pipeline is the same for Germline events:

  27. List of useful websites:especially for variant analysis • http://samtools.sourceforge.net/ • http://picard.sourceforge.net/ • http://www.broadinstitute.org/gatk/ • http://seqanswers.com/ • http://www.biostars.org/ • For GERMLINE studies • http://www.1000genomes.org/ • http://hapmap.ncbi.nlm.nih.gov/ • For Cancer • http://cancergenome.nih.gov/ Not even close to a comprehensive list; just some jumping off points and must see places

  28. Side Note • Bioinformatics is very much a science of the internet • Much of the “knowledge” is not in “paper” (published) form • Need to read blogs as much as journals • Google: • perhaps one the most important tools for bioinformatics research • Both blessing and curse

  29. State of the Art for Variant Dectection • GATK pipeline form the Broad • At MSKCC have pipelines that use both • GATK 1.6 branch • new GATK 2.x branch

  30. non-GATK Box • Actually pretty complex

  31. Research Pipeline Detailed

  32. non-GATK Box • 3 (4) key steps • Adapter clipping (FASTX or cutadapt) • Mapping to genome (BWA: genome issues) • SAM/BAM massaging • Add ReadGroups (PICARD) • Sort by coordinates at this step • MarkDuplicates (PICARD) • Filter on MAPQ

  33. Which mapper • There are many; we have settled on BWA because the pros seem to use it more widely • GATK 1000g • TCGA • Many mappers do not compute MAPQ which our algorithms need

  34. Genome • Some controversy/discussion over what to use when mapping • For SNP detection key is to not have misplaced reads due to homologous regions not in main build • Map to all chromosomes include • random (unplaced) • unassigned • decoy genome • http://www.cureffi.org/2013/02/01/the-decoy-genome/ • everything but the haplotype chromosomes

  35. Intermediate Output BAM • BAM is a standard format for representing alignments. • A very useful tool for visualizing BAM’s is IGV • http://www.broadinstitute.org/igv/

  36. GATK Stuff • Currently we stick as closely as possible to the:Best practices guidelines from GATK: • http://www.broadinstitute.org/gatk/guide/topic?name=best-practices • Currently at version 4 • STRONGLY, encourage people to go through slides from GATK BP series. • Much of what I show hear is an excerpt from those. • Send someone to one the Broad courses.

  37. GATK Stuff • Lots of stuff; cover here 3 critical steps • In/Del Realignment • BaseQ Recalibration • Variant Calling

  38. Realignment • InDels in reads (especially near the ends) will often be miss-aligned by most mappers as reads with mismatches. • These false mismatches can degrade base quality recalibration and lead to false positives SNP calls

  39. Base Quality Recalibration • The Q-score is a measure provided by the machine of how accurate “it” thinks it read the base: • Q = -10*log10(Perror) • referred to has PHREAD score • Lots and lots of problems with the vendors estimate of this value • Different vendors are not even on the same scale sometimes • Same vendor is not on same scale • Illumina FASTQ debacle

More Related