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Rogalski T.M., Mullen G.P., Gilbert M.M., Williams B.D., and D.G. Moerman

The UNC-112 gene in Caenorhabditis elegans Encodes a Novel Component of Cell-Matrix Adhesion Structures Required for Integrin Localization in the Muscle Cell Membrane. Rogalski T.M., Mullen G.P., Gilbert M.M., Williams B.D., and D.G. Moerman Journal of Cell Biology, 150:253-264. The Authors.

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Rogalski T.M., Mullen G.P., Gilbert M.M., Williams B.D., and D.G. Moerman

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  1. The UNC-112 gene in Caenorhabditis elegans Encodes a Novel Component of Cell-Matrix Adhesion Structures Required for Integrin Localization in the Muscle Cell Membrane Rogalski T.M., Mullen G.P., Gilbert M.M., Williams B.D., and D.G. Moerman Journal of Cell Biology, 150:253-264

  2. The Authors Teresa Rogalski Research Scientist Department of Zoology Ph.D., Simon Fraser Gregory Mullen Department of Zoology UBC Mary Gilbert Department of Zoology UBC Donald G. Moerman Professor B.Sc,, Ph.D., Simon Fraser Postdoc, Washington University School of Medicine Visiting Scientist, Max-Planck Institute for Biochemistry Benjamin D. Williams Associate Professor of Cell & Structural Biology University of Illinois Sc.B., Brown University (Biology) Ph.D., Yale University (Biology) Postdoc., Washington University School of Medicine

  3. Muscle in C. elegans

  4. Dense Bodies in C. elegans This paper focuses on `s interactions with perlecan, vinculin, and ß-integrin UNC-112

  5. UNC-112 and its neighbors Perlecan: located in the basement membrane Vinculin: binds actin & a-actinin ß-integrin: major structural TM component

  6. Dense Bodies Why are important? Muscle fibers need anchors …….. in order to contract!

  7. Dense Bodies in C. elegans

  8. Muscle Assembly during Development nearly continuous sheet forms focal adhesions ..the sheet seperates into muscle quadrants

  9. UNC-112 Is a good neighbor? Perlecan ---> <--- ß-integrin <-- FA Vinculin --->

  10. UNC-112 and UNC-52 / perlecan wild type (at 3-fold) unc-112(st581) (PAT) Adhesion Structures

  11. UNC-112 and DEB-1 / vinculin wild type (at 3-fold) unc-112(st581) (PAT) Adhesion Structures

  12. UNC-112 and PAT-3 / ß-integrin wild type (at 3-fold) unc-112(st581) (PAT) Adhesion Structures

  13. UNC-112 and PAT-3 / ß-integrin UNC-112::GFP colocalizes with PAT-3 immunofluorescence

  14. UNC-112 lends a hand.. • Required for proper ß-integrin localization • Is needed to organize vinculin & perlecan* • However, vinculin does not need UNC-112 to localize itself with ß-integrin *but not required.

  15. UNC-112 in wild type embryo close-up of UNC-112::GFP Myosin light-chain mAb UNC-112::GFP

  16. UNC-112 in unc-44(-) / deb-1(-) (vinculin mutant) close-up of UNC-112::GFP Myosin light-chain mAb UNC-112::GFP

  17. UNC-112 in unc-52(-) embryo (perlecan mutant) close-up of UNC-112::GFP Myosin light-chain mAb UNC-112::GFP

  18. UNC-112 in pat-3(-) embryo (ß-integrin mutant) close-up of UNC-112::GFP Myosin light-chain mAb UNC-112::GFP

  19. UNC-112 needs a little help.. • Requires ß-integrin & perlecan for activation • Without them, cannot anchor FA complexes • Myosin is hopelessly lost without UNC-112

  20. UNC-112 Human Ortholog of (kindlin-1) …also plays a role in proper attachment of actin to vinculin

  21. UNC-112 Human Ortholog of (kindlin-1) Otherwise……

  22. UNC-112 Summary • Not required for: • -- Polarization of muscle cell membrane • -- Focal Adhesion (FA) complex formation • Required for: • - Neat and orderly arrays of FA complexes • -- Proper attachment of actin to FAs • - Any Attachment of Myosin to FAs

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