'Target sequence' presentation slideshows

Real-Time PCR M.Tevfik DORAK, MD PhD updated on April 3, 2012 dorak

Real-Time PCR M.Tevfik DORAK, MD PhD updated on April 3, 2012 www.dorak.info. Cockerill FR III. Arch Pathol Lab Med. 2003;127:1112 (www). What is Wrong with Agarose Gels?. * Poor precision * Low sensitivity * Short dynamic range < 2 logs * Low resolution * Non-automated

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Computational Analysis of Proteins

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Blast

Blast. Review. Basic Local Alignment Search Tool Initially developed and used to search genomic sequence Ex) I have a short piece of mRNA/EST that I sequenced -- and I'd like to see where it is in a genome Especially useful before genome browsers

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Chapter 8 Mutagenesis As a Genomic Tool For Studying Gene Function

Chapter 8 Mutagenesis As a Genomic Tool For Studying Gene Function . Tian Baoxia 2011. 04. 06. Introduction.

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In Vivo assay for RNA-protein interactions

In Vivo assay for RNA-protein interactions. Dana M. Schneider Loren Williams lab. Outline. The Ribosome Yeast three hybrid assay for RNA-protein interactions Construct vectors PCR amplify insert Restriction digest plasmids and insert Ligate vector and insert

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Biomolecular Modeling (practical)

Biomolecular Modeling (practical). Dr. Kong Lesheng Temasek Life Sciences Laboratory (TLL) (lesheng@tll.org.sg). Objective. To predict 3D structure with good quality when given a protein sequence. Protein structure prediction. Comparative (homology) modeling typically >20-30% identity

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Loop-mediated Isothermal Amplification (LAMP) and its application in detection A. Ishwara Bhat

Loop-mediated Isothermal Amplification (LAMP) and its application in detection A. Ishwara Bhat Senior Scientist Indian Institute of Spices Research Marikunnu , Calicut 673012. Loop mediated isothermal amplification (LAMP)

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Bioinformatics Part 3: Pairwise Alignments and Database Searches

Bioinformatics Part 3: Pairwise Alignments and Database Searches. Similarity, homology and identity Gap penalties and scoring matrices in pairwise alignments Alignment algorithms Database searching: BLAST and FASTA. Similarity, Homology and Identity.

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The Polymerase Chain Reaction

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Molecular Testing and Clinical Diagnosis

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BACTERIAL TRANSPOSONS

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Individual sequences in large sets of gene sequences may be distinguished efficiently by combinations of shared sub-seq

Individual sequences in large sets of gene sequences may be distinguished efficiently by combinations of shared sub-sequences. Mark J Gibbs, John S Armstrong and Adrian J Gibbs BMC Bioinformatics 2005, 6:90 Presented by Miguel Gonzalez. Outline. Background Results Discussion. Background.

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Genetic Technologies

Genetic Technologies. By: Ting-Yu Tsai Han Keyl Kim. 19.1 Patenting DNA. Biotechnology -Use or alteration of cells or biological molecules for specific applications. Organisms that harbor DNA from other species are termed Transgenic and their DNA is called recombinant DNA.

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Paired Box Domains

Paired Box Domains. MRCPath Self Help 2008. Identification of Paired Box Domain. First identified in the Drosophila pair-rule segmentation gene paired and the two segment polarity genes gooseberry-distal and gooseberry-proximal

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Molecular viewers and protein structure prediction

Learn how to display and manipulate protein structures with Deep View. Workshop-Learn how to use Deep View molecular modeler. Protein structure viewers. RasMol ...

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Local alignment and BLAST

Local alignment and BLAST. Usman Roshan BNFO 601. Local alignment. Global alignment recursions: Local alignment recursions. Local alignment traceback. Let T(i,j) be the traceback matrices and m and n be length of input sequences. Global alignment traceback:

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