'Rna samples' presentation slideshows

Southern, Northern and Western blotting

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Tools

Tools. Enzymes Vectors Host DNA to be cloned. Enzymes. Nucleases Polymerases Ligases , Modifying enzymes Topoisomerase , . Exonucleases. Unit Enzyme 1ug DNA at 37C in 60 min in 50 ul reaction* ExonucleaseI ssDNA 3-5 ExonucleaseIII dsDNA 3-5

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Microarray Platform Comparison RoundTable Discussion

Microarray Platform Comparison RoundTable Discussion. Participants: Barry Merriman, UCLA Dept of Human Genetics Peter Herzer, Phd., Amersham Bioscience Applications Consultant Damon Harvey, Affymetrix field applicaions scientist James Willey, M.D. -Medical College of Ohio

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Introduction to microarray technology

Introduction to microarray technology. Lecture 17, Statistics 246 March 18, 2004. Outline. A little background Types of microarrays cDNA arrays Affymetrix GeneChips. Uses and types of microarrays.

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Design of Micro-arrays

Design of Micro-arrays. Lecture Topic 6. Experimental design. Proper experimental design is needed to ensure that questions of interest can be answered and that this can be done accurately , given experimental constraints, such as cost of reagents and availability of mRNA.

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Microarray analysis

Microarray analysis. Golan Yona ( original version by David Lin ). What makes one cell different from another? liver vs. brain Cancerous vs. non-cancerous Treatment vs. control. There are about 100,000 genes in mammalian genome each cell expresses only ~15,000 of these genes

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BIT 815: Analysis of Deep Sequencing Data

BIT 815: Analysis of Deep Sequencing Data. Overview:

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FISH ( FLUORESCENCE IN SITU HYBRIDISATION)

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Cardiac Morphology, Gene Expression and Histology (Core D)

Cardiac Morphology, Gene Expression and Histology (Core D). Description of Core Unit: Generate staged mouse and Xenopus embryo RNA samples. Generate paraffin and OCT embedded tissues and embryos. Share expertise in and perform some whole mount and section in situ hybridizations.

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1471-2164-14-292-s1

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1471-2164-14-292-s8

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