Quantitative proteomics. Peking Union Medical College Chinese Academy of Medical Sciences Wei Sun email@example.com. Content. 1.Introduction 2. MS-based methods 3. Gel-based methods. Petterson SD, et al. Nat Genetics, 2003, 33, 311-23.
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Brief Introduction of Yeast Two-hybrid System. Xiaoqin Lai Dec. 2, 2004. Dimensions of Information Complexity Genomics vs. Post-Genomics. Protein Interaction. 10 6. Human Proteome. 10 5. Transcripts. Human Genome. Gen ome : 30.000 genes Transcript ome: 40-100.000 mRNAs
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Proteins are dynamic systems. Concerted motions of the p53 binding domain of MDM2. protein dynamics: Timescale: from s to fs. Inhomogeneity within same structure. Conformational substates Temperature dependence. Motions of tyrosine kinase. Observation of protein dynamics.
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MATHEMATICAL PROPERTIES OF NETWORK OF PROTEIN INTERACTIONS . Abhishek Rathod CS 374 Algorithms in Biology Prof. Serafim Batzoglou . Broad plan . Part A Basics of Protein Interaction Networks and some background
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Introduction. ??? National Taipei University of Technology Feb 24, 2003. Outline. Introduction to proteomics Definitions of Proteomics The major techniques in current proteomics Protein-protein interaction Major Directions in Coming Proteomics. Outline. Introduction to proteomics
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Tutorial: Bioinformatics Resources. ( http://pir.georgetown.edu/pirwww/workshop/bioinfo_resource.html ). Bio-Trac 25 (Proteomics: Principles and Methods) October 5, 2007 Zhang-Zhi Hu, M.D. Research Associate Professor Protein Information Resource, Department of
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Protein-protein Interactions and Pathways. June 26, 2014. Why PPI?. Protein-protein interactions determine outcome of most cellular processes Proteins which are close homologues often interact in the same way
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BCB 444/544. Lecture 20 Protein Structure Basics, Visualization, Classification & Comparison #20_Oct08. Required Reading ( before lecture). Mon Oct 8 - Lecture 20 Protein Secondary Structure Prediction Chp 14 - pp 200 - 213 Wed Oct 10 - Lecture 21
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MSD pisa a web service for studying P rotein I nterfaces, S urfaces and A ssemblies Eugene Krissinel. http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html. What PISA is about. Crystal = translated Unit Cell.
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Molecular Biology Bio4751 Spring 2003 Gary A. Bulla, PhD. Methods in Molecular Biology. RNA quantitation. a. Northern. 1. Lyse with detergent, 2. Phenol extract (removes proteins) 3. Precipitate RNA 4. Load onto agarose gel 5. Transfer RNA to nylon membrane
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Hanahan & Weinberg: The hallmarks of cancer. Cell 100:57-70, 2000. ONCOGENE ACTIVATION. ONCOGENE ACTIVATION. TUMOR SUPPRESSOR INACTIVATION. 1) Point mutation. p53. TUMOR SUPPRESSOR INACTIVATION. 1) Point mutation : p53, BRCA1 etc. 2) Deletion 3) Epigenetic silencing. Locus 9p21.
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3-1. Protein folding in the cell (I). Basics - cell compartments, molecular crowding: cytosol, ER, etc. Folding on the ribosome - co-translational protein folding Molecular chaperones - concepts, introduction - intramolecular chaperones - chemical chaperones - protein chaperones. 3-2.
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LECT 22: LIPIDS, MEMBRANES, AND CHOLESTEROL. Lipids and cholesterol in the body derive from food intake and from de novo synthesis. Phospholipids are principle constituent of cell membranes, and specialized phospholipids participate in signal transduction pathways.
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Protein domains vs. structure domains - an example. Genome of the week. Nanoarchaeum equitans - archaea Hyperthermophile Diverged early in evolution from other archaea New kingdom of archaea? Obligate symbiont with Ignicoccus Smallest completely sequenced genome <500kB
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The Two-Hybrid Method for Identifying and Analyzing Protein-Protein Interactions. TAD. TAD. Sst2 interacts with Ste2 in a DEP-dependent manner in vivo. nucleus. Ub C-terminal hydrolase. NubG. HIS3. TAD. HIS3. TAD. HIS3. HIS3 encodes imidazoleglycerol-phosphate dehydratase
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Approaches to signal transduction: - pathway - ligand/receptor - protein/protein interactions - second messengers - protein phosphorylation - cellular responses Shutting down a signaling pathway. (second messengers). Approaches for identifying a pathway:
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