'Log 2 cy5 cy3' presentation slideshows

Microarray Data Normalization and Analysis

Microarray Data Normalization and Analysis. John Quackenbush 15 February 2003 MGED/AAAS Denver. The Experimental Design. The Experimental Design dictates a good deal of what you can do with the data Good normalization and processing reflects the experimental design

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Analysis of Affy 1.0 ST Gene Array Data in R

Analysis of Affy 1.0 ST Gene Array Data in R. To analyze Affymetrix 1.0 ST data (exon or gene) you need: Expression data in .CEL format A CDF (chip definition file) that is appropriate to the array platform (different for exon and gene arrays!) The affy package for R

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The following slides have been adapted from tm4/ to be presented at the

The following slides have been adapted from http://www.tm4.org/ to be presented at the Follow-up course on Microarray Data Analysis (Nov 20-24 2006, PICB Shanghai) by Peter Serocka. MIcroarray Data Analysis System (version 2.19 ). Wei Liang October 2004. Printer. Scanner. Database.

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MIcroarray Data Analysis System (version 2.19 )

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MIcroarray Data Analysis System (version 2.19 )

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Microarray hybridization

samples. mRNA. cDNA. DNA microarray. Microarray hybridization. Usually comparative Ratio between two samples Examples Tumor vs. normal tissue Drug treatment vs. no treatment Embryo vs. adult. 2. Spotting DNA on the chip. Types of printing pins. Apply small droplets on contact.

1.81k views • 24 slides