'Ldl protein profiling' presentation slideshows

The influence of insulin resistance on very low-density lipoprotein proteome changes in type 2 diabetes after oral high-

Insulin resistance plays a role in diabetic dyslipidaemia, especially postprandial hypertriglyceridaemia, through the overproduction of very low-density lipoprotein (VLDL). However, the role of insulin resistance on the apoprotein profile of VLDL has not been explored. To study the influence of insulin resistance on the VLDL proteome in the fasting and postprandial states. Eleven type 2 diabetes (T2DM) subjects with moderated metabolic control (HbA1c <8%) were classified as lower insulin resistance, LIR (HOMA-IR<2.5, n=5), and higher insulin resistance, HIR (HOMA-IR 2.5-5.0, n=6), and were compared to 10 non-diabetic controls. After 12 hours of fasting, the subjects consumed a fat-rich meal. Both before the meal and 4 hours after the meal, blood samples were taken to study the VLDL proteome. VLDL-associated proteins were separated and quantified with twodimensional gel electrophoresis (2D-GE) and identified using mass spectrometry.

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