'Gel filtration' presentation slideshows

Chapter 5 Molecular Tools for Studying Genes and Gene Activity

Chapter 5 Molecular Tools for Studying Genes and Gene Activity. Jay D. Hunt, Ph.D. Department of Biochemistry and Molecular Biology CSRB 4D1 568-4734 jhunt@lsuhsc.edu. Electrophoresis Agarose gel electrophoresis. Separates DNA (or RNA or Protein) fragments on the basis of charge and size

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Lecture 8: Protein purification

Lecture 8: Protein purification. Protein purification. Column chromatography. After the initial fractionation steps we move to column chromatography . The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase .

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Figure 5-60

Figure 5-60. Page 122. EcoRI. Tetracycline Resistance. Ampicillin Resistance. PstI. HindIII. +. +. +. +. +. Origin of Replication. A plasmid. Figure 5-43 The pUC18 cloning vector. Page 106. Figure 5-46 Construction of a recombinant DNA molecule. Page 108.

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Types of Centrifuges used to measure the S-value

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Protein Purification Lab C2 Pages 115 to 168

Protein Purification Lab C2 Pages 115 to 168. Lab C.2 Four Periods Protocol Page 135-160 Benchtop Protocols begin on page 398 Be sure to read theory starting page 120. Exam. Exam March 3,4 Includes Carbohydrates, Enzyme kinetics, and all protein labs and material related there to.

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Electrophoresis

Electrophoresis. Separation, analytical or preparative, of charged molecules by migration through a matrix due to application of an electric field, with net movement towards electrode of opposite charge. General Principle.

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Isolation and Characterization of alpha-Lactalbumin

Isolation and Characterization of alpha-Lactalbumin. Experiment#4 Week#1--Protein purification using Gel Filtration. Protein Separation/ Purification. Purpose : examine techniques in purifying ? -Lactalbumin, a constituent in milk alpha-Lactalbumin: MW= 15,500 Daltons

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Techniques of Protein Purification

Chapter 6. Techniques of Protein Purification. You should become familiar with this entire chapter so that you can use it for reference. But you are only responsible for the topics in the chapter that were covered in lecture. Protein isolation Selection of protein source

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BICH 605

BICH 605. Protein Purification. Sample Preparation Extraction (grinding, detergent lysis, sonication) Salt exchange (gel filtration, filters, dialysis) Capture Ion Exchange Affinity Hydrophobic Interaction Intermediate Purification Ion exchange Hydrophobic Interaction Polishing

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Proteoma

Proteoma. Milk, a source of nourishment for all mammals, is composed, in part, of a variety of proteins. The protein components of milk are revealed by the technique of MALDI-TOF mass spectrometry, which separates molecules on the basis of their mass to charge ratio. OD 340nm.

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Measuring the size and shape of macromolecules

Measuring the size and shape of macromolecules.

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Downstream Processing

Downstream Processing. Know the Characteristics of Your Protein Green Fluorescent Protein (GFP). Sequence of Amino Acids. Tertiary Structure.

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PiMS at the OPPF

PiMS at the OPPF. Jon Diprose EMBO Course EBI, 23/09/2008. Overview. The OPPF Workflows and Protocols Using PiMS at the OPPF. Aims of the OPPF. To develop technology for high throughput protein production and crystallisation.

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Chromatography

Chromatography. Desalting and Affinity. Chromatography. Technique to separate components of a mixture by passing them through a matrix. A solvent is used for carrying sample mixture through the matrix Separation occurs because each compound in a mixture interacts differently with the matrix.

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Separation techniques

Separation techniques. Lineout. Motivation Precipitation Sedimentation Solvent-controlled precipitation Density gradient centrifugation Solvent extraction Dialysis Filtration Microfiltration Ultrafiltration Gel electrophoresis Chromatography

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Gel filtration chromatography

Gel filtration chromatography. http://higheredbcs.wiley.com/legacy/college/voet/0471214957/animated_figures/ch05/f5-6.html. Gel filtration (chromatography), is also known as molecular sieve chromatography. Gel filtration chromatography separates molecules according to their size and shape.

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Size Exclusion Chromatography

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Proteoma

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Radioimmunoassay (RIA): A Remarkably Sensitive Bioassay

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Class overheads for Protein Homogeneity, CHEM 645

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