Scientific Reading and Writing in English Results Shu-ying Wang, Ph.D. Dept. Microbiology and Immunology Tel: +886-6-2353535 ext. 5634 Fax: +886-6-2082705 Email : firstname.lastname@example.org.By harlow
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DNA Enzyme reactions. Ligation Restriction enzymes Helicase enzymes Polymerization Strand-displacing polymerases. Big Picture - Enzymes. Exonucleases Endonucleases Restriction Endonucleases Type I – cut elsewhere of recognition sites Type II – cut within recognition sites.
Restriction Enzyme Digestion of DNA. WHAT ARE RESTRICTION ENZYMES?. Restriction Enyzmes – molecular scissors able to cut DNA. Discovering Restriction Enzymes. Werner Arber Daniel Nathans Hamilton Smith.
Restriction Enzyme Digestion of DNA. Experiment Goals. Digestion of DNA by restriction enzyme Analyze digested DNA by electrophoresis. Restriction Enzymes.
Restriction Enzyme Cleavage of DNA . Carolina Kit. Timeline. Week Before: HW—DNA scissors, DNA goes to the races Monday—pour gels Tuesday—Lecture, Run gels with DNA, photograph gel Monday—Lab write-up due. Write-up. Annotate handout
Biotechnology: Restriction Enzyme Analysis of DNA. AP Biology Investigation 9 Gel Electrophoresis Lab. Introduction. Genetic Engineering : process of manipulating genes and genomes Biotechnology : process of manipulating organisms or their components for the purpose of making useful products.
DNA-based Tools. ASO (Allele Specific Oligonucleotides) Used to detect specific alleles. Can determine if a person is homozygous or heterozygous for a particular allele. DNA-based Tools. ASO (Allele Specific Oligonucleotides)
DNA Based Biosensors. Yingli Fu Biological Resources Engineering University of Maryland, College Park December 10, 2003. Outline. Introduction Principles of DNA biosensors Types of DNA biosensors Improvement of DNA biosensors DNA biosensor miniaturization References. Introduction.
Restriction Enzyme digestion of DNA - Exercise 8. Objectives -Understand how Restriction Enzymes digest DNA. -Know how to construct a pAMP (plasmid) or gel. -Given the size of fragments, gel, know how to construct a restriction map. -Given a restriction map know how to construct a gel.