'Control sirna' presentation slideshows

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Cortical plaques

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G-Fectin TM

G-Fectin TM. 1. A novel transfection reagent developed by Genolution 2. Cationic lipid based 3. no cell splitting is required 4. Whole transfection can be done in 10 min 5. Your gene silencing in 24 hours 6. Lower toxicity than other reagents Better gene silencing

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Control siRNA BRG1 siRNA

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Viability (% control)

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Control siRNA

A. B. c-MYC siRNA. c-MYC siRNA. c-MYC siRNA. Control siRNA. Control siRNA. Control siRNA. c-MYC siRNA. Control siRNA. 250. 200. 150. p21 protein expression (% control). 100. 50. 0. p21 (21 kDa). ? -Actin (42 kDa). D341. D425. DAOY wt. DAOY M2. DAOY V11. DAOY wt. 1. 0.8.

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HBO1 siRNA #2

Supplementary Figure 1. A. E. C. HeLa cells. control siRNA. Whole cell extracts. Chromatin fractions. Input (5%). IP : HBO1. IP : IgG. %G1/G0 %S %G2/M. 41.70 26.56 31.74. -. -. -. -. +. +. HBO1 siRNA #2. 200. Number of cells. -. -. -. -. +. +. Control siRNA. 100.

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7-AAD

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14-3-3 ?

P-Akt. 14-3-3 ?. Control siRNA. 14-3-3 ? siRNA. Neal et al.Supplementary Figure S1. a. b. Control siRNA. 14-3-3 ? siRNA. C1 C2 si1 si2 si3. P-Akt. Akt. 14-3-3 ?. c. d. Control siRNA. 14-3-3 ? siRNA. Control shRNA. 14-3-3 ? shRNA1. 14-3-3 ? shRNA2. 0.

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0

*. *. *. *. *. A. *. Relative apoptosis. Relative proliferation. 1. 2. miR-138 mimic control mimic anti-miR-138 LNA control LNA FOSL1 siRNA control siRNA. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. +. +. +. +. 1. 0.5. -. -. -. -. -. -. -. -.

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Control siRNA

sonicated DNA. 100bp ladder. (bp). 2000. 1500. 1000. 500. 100. a. c. 1.2. 1.0. 0.8. Relative EGR-1 mRNA. 0.6. 0.4. 0.2. 0. Mock. EGR-1 siRNA. b. EGR1 siRNA. Control siRNA. Additional data file 1.

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Yeom Supplementary Fig.4

Yeom Supplementary Fig.4. siControl siRRAD#1 siRRAD#2. RRAD. pSTAT3(Y705 ). TWIST. b - actin.

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***

A. *. ns. B. ***. *.

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