Effects of Etoposide and Cisplatin on Viability of DAOY Cells with c-MYC siRNA Interventions
This study investigates the impact of Etoposide and Cisplatin on the viability of DAOY cells, utilizing c-MYC siRNA for targeted intervention. Various concentrations of the drugs were tested, and cell viability was assessed as a percentage compared to control. The results indicate that c-MYC silencing may modulate the response of DAOY cells to chemotherapeutic agents, providing insights into potential therapeutic strategies for medulloblastoma treatment. Statistical analysis was performed to confirm the significance of the results.
Effects of Etoposide and Cisplatin on Viability of DAOY Cells with c-MYC siRNA Interventions
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A DAOY wt Etoposide µg/ml Cisplatin µg/ml c-MYC siRNA c-MYC siRNA Control siRNA Control siRNA 120 120 100 100 80 80 60 60 40 40 20 20 0 0 120 100 80 Viability (% control) 60 40 0.6846 [0.5360 - 0.8744] 20 0.7464 [0.6653 to 0.8372] 0 0 0.08 0.15 0.30 0.60 1.20 2.40 4.80 0.04 DAOY V11 D341 120 100 0.5166 [0.3348 - 0.7973] 80 Viability (% control) 0.2558 [0.1048 - 0.6245] Viability (% control) 60 40 0.4631 [0.4045 to 0.5302] 20 0.4929 [0.4567 to 0.5320] 0 0 5 0 40 10 20 0.16 0.31 0.63 1.25 2.50 4.80 0.04 0.08 0.15 0.30 0.60 1.20 2.40 DAOY M2 D425 120 100 80 Viability (% control) Viability (% control) 60 40 0.7143 [0.5789 - 0.8813] 3.195 [2.902 - 3.517] 20 0.182 [0.09133 - 0.3642] 1.854 [1.390 - 2.474] 0 0 5 0 10 20 40 0.16 0.31 0.63 1.25 2.50 0.04 0.08 0.15 0.30 0.60 1.20 2.40 4.80 B DAOY wt 120 100 80 Viability (% control) 60 40 1.225 [1.014 - 1.480] 20 0.259 [0.2043 - 0.3290] 0 5 0 0.04 0.08 0.16 0.31 0.63 1.25 2.50 DAOY V11 D341 120 120 100 100 80 80 Viability (% control) Viability (% control) 60 60 40 40 3.184 [2.511 - 4.037] 0.112 [0.1016 - 0.1237] 20 20 0.516 [0.3769 - 0.7063] 0.058 [0.04713 - 0.07204] 0 0 5 0 0 0.04 0.08 0.16 0.31 0.63 1.25 2.50 1.25 0.01 0.02 0.31 0.63 0.04 0.08 0.16 DAOY M2 D425 120 120 100 100 80 80 Viability (% control) Viability (% control) 60 60 40 40 2.627 [1.669 - 4.134] 3.80 [1.947 - 8.415] 20 20 0.182 [0.09133 - 0.3642] 2.03 [1.091 - 3.770] 0 0 0 5 0 5 10 20 40 0.04 0.08 0.16 0.31 0.63 1.25 2.50 0.16 0.31 0.63 1.25 2.50
