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CRISPR Cas9 Genome Editing in Germlines

CRISPR Cas9 Genome Editing in Germlines

CRISPR Cas9 Genome Editing in Germlines. PBIO 4500 December 5, 2017 Sarah Disney. What is CRISPR Cas9?. Discovered in E. coli CRISPR = Clustered Regularly Interspaced Short Palindromic Repeats An adaptive bacterial immune defense

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284 views • 9 slides
Knockout Cell

Knockout Cell

CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing. It has been widely used in current biology, functional genome screening, cell-based human hereditary disease modeling, epigenomic studies and visualization of cellular processes. https://www.creative-biogene.com/Services/Stable-cell-line-generation/Custom-Genome-Editing-Cell-Lines.html

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58 views • 4 slides
Gene Synthesis

Gene Synthesis

Gene synthesis is the process of chemically synthesizing double-stranded DNA molecules in vitro. It differs from molecular cloning and polymerase chain reaction (PCR) in that the researchers do not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. https://www.creative-biogene.com/Services/Gene-Service/Gene-Synthesis-and-cloning-service.html

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49 views • 4 slides
Gene Synthesis

Gene Synthesis

Gene synthesis is the process of chemically synthesizing double-stranded DNA molecules in vitro. It differs from molecular cloning and polymerase chain reaction (PCR) in that the researchers do not have to begin with preexisting DNA sequences. https://www.creative-biogene.com/Services/Gene-Service/Gene-Synthesis-and-cloning-service.html

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49 views • 4 slides
Gene Synthesis

Gene Synthesis

Gene synthesis is the process of chemically synthesizing double-stranded DNA molecules in vitro. It differs from molecular cloning and polymerase chain reaction (PCR) in that the researchers do not have to begin with preexisting DNA sequences. https://www.creative-biogene.com/Services/Gene-Service/Gene-Synthesis-and-cloning-service.html

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49 views • 4 slides
Gene Synthesis

Gene Synthesis

Gene synthesis is the process of chemically synthesizing double-stranded DNA molecules in vitro. It differs from molecular cloning and polymerase chain reaction (PCR) in that the researchers do not have to begin with preexisting DNA sequences. https://www.creative-biogene.com/Services/Gene-Service/Gene-Synthesis-and-cloning-service.html

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43 views • 4 slides
Gene Synthesis

Gene Synthesis

Gene synthesis is the process of chemically synthesizing double-stranded DNA molecules in vitro. It differs from molecular cloning and polymerase chain reaction (PCR) in that the researchers do not have to begin with preexisting DNA sequences. https://www.creative-biogene.com/Services/Gene-Service/Gene-Synthesis-and-cloning-service.html

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49 views • 4 slides
knockout cell lines

knockout cell lines

The CRISPR/Cas9 system is an adaptive immunity system in bacteria against bacteriophage invading. It has been developed as the most popular method for genome editing in recent years. https://www.creative-biogene.com/Product/Gene-Knockout-Stable-Cell-Lines.html

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53 views • 4 slides
CRIPSR-Cas9?

CRIPSR-Cas9?

Researchers from Harvard Medical School researchers set up spCas9 variants. The spCas9 variants can target sites in human and zebra fish genes more precisely, which is not modified by the original one.

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251 views • 1 slides
Breakthrough Technology to Improve the Range and Accuracy of Cas9 Target

Breakthrough Technology to Improve the Range and Accuracy of Cas9 Target

Scot A Wolfe, an associate professor of molecular, cell and cancer biology at the University of Massachusetts School of medicine, reported that they developed a new technology that could significantly improve the accuracy of Cas9 and expand its target range by fusing the DNA binding domain (pDBD) and CRISPR/Cas9 System.

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163 views • 1 slides

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