'5 ml min' presentation slideshows

renal training

renal training. Judith Coombes Pharmacist Princess Alexandra Hospital And School of pharmacy University of Queensland Material provided by Jo Sturtevant Renal and Transplant pharmacist Princess Alexandra Hospital and Graham Davies, Kings College London.

650 views • 45 slides

Principles of Flow Chemistry

428 views • 20 slides

Nanowires and Nanorings at the Atomic Level

286 views • 15 slides

Metabolite Profiling of Fruit and Seed Extracts of Garcinia xanthochymus using RpHplc- Esi-Q-Tof-Ms and Progenesis Qi

Natural products research is the most enormous field of research in terms of the amount of data and importance of information. Natural products discovery and metabolomics deals with a crucial mode of representation of the profile of biologically active metabolites. In this regard, the profiling of the chemical makeup of complex natural plant extracts necessarily requires employing sophisticated and advanced analytical methods like RP-HPLCu2013ESI-Q-TOF-MS as well as data mining and processing methods. The genus Garcinia (Clusiaceae) contains phenolic, flavonoids, xanthones, triterpenes, and benzophenones which have been reported for their significant biological properties. Due to its high content of secondary metabolites and its large domestic usage, we have developed a simple, rapid and precise method to characterize all the secondary metabolites using Reverse-Phase Ultra Performance Liquid Chromatography coupled to Electrospray Ionization Quadruple Time-of-Flight Mass Spectrometry (RP-HPLCu2013ESI-Q- TOF-MS) for the hydromethanolic extract. A total of about 3443 secondary metabolites from the fruit and 3757 secondary metabolites from the seed were identified by the Progenesis-QI data analysis. Among these a total of 74 compounds from fruits and 86 polar compounds from seeds were manually identified using the mass error limit of < u00b1 5 ppm including the score less than 40. The unexplored bioactives belonging to the class of glycosides, flavones, xanthones, organic acids and other phenolic derivatives. Garcinia xanthochymus was found to contain significant number of diverse phytochemical components. These results indicate the profile of molecules present in G. xanthochymus and will be helpful for industries and researchers involved in isolation of their molecules of interest.

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GC setup

GC setup. 2 ml/min. 2 ml/min. 2 ml/min. 103 ml/min. 28 ml/min. 28 ml/min. 24 ml/min. 25 ml/min. 100 ml/min. Split ratio 25:1. Split ratio 24:2. Split ratio 100:1. 2 ml/min. 1 ml/min. 1 ml/min. SPLIT Injections. www.kinesis-solutions.co.uk/kinesis_techtips.asp?id=11.

243 views • 13 slides

Solid phase extraction of copper, cadmium and lead in environmental samples FAAS using activated carbon modified with 5-

Sensitive and selective preconcentration procedure was developed for the determination of trace Cu(II), Cd(II) and Pb(II) ions in environmental samples. 5(4-dimethyl aminobezyledeneamino) 2-hydroxy benzoicacid modified with activated carbon was prepared and used as new solid phase extractor. Adsorbed metal ions were eluted with 10ml of 3N HNO3 at a flow rate of 2 ml/min. The concentration of metal ion determined by flame atomic absorption spectrometry (FAAS). Different factors including pH of the sample solutions, effect of sample volume, the amount of modified activated carbon, type and volume of eluent were examined. The proposed method was applied to the determination of metal ions in water samples and soil samples with satisfactory results (recoveries range from 96 to 99%).

95 views • 9 slides