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Comprehensive Study on Ectopic HCVc174 Protein Interactions

This study explores the interaction of HCVc174 protein with key candidates through affinity purification, comparative SDS-PAGE, LC-MS/MS analysis, and confirming RNA-dependent interactions. Additionally, it delves into subcellular distributions and HCVc174 cytoplasmic confinement during viral replication.

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Comprehensive Study on Ectopic HCVc174 Protein Interactions

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  1. 1. Expression of ectopic HCVc174 with fusion tags 2. Affinity purification and comparative SDS-PAGE for identification of prominent differential protein bands 4. Confirmation of hnRNPH1, NF45, and C14orf166 as HCVc174-interacting candidates 7. Cytoplasmic confinement of the 3 candidates with HCVc during Con1-1b or JFH1-2a replication 3. LC-MS/MS analysis of 10 selected protein bands for HCVc174-interacting candidates 6. Subcellular distributions of the 3 candidates and prominent nuclear co-localization of HCVc174 5. Interaction of HCVc174 and NF45 is RNA-dependent

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