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Explore the intricate genetic replication and transcription mechanisms in eukaryotic organisms, including minus-strand priming sites, plus-strand primers, polyadenylation consensus sequences, splice donor and acceptor sites. Uncover the details of genetic processes crucial for cellular functions.
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a. Replication (i) Minus-strand priming site (ii) Plus-strand primer 4321 CGCCCGCCATTATTTTCAAACATTCCTGAAA 4329 ............................... 4327 ............................... 4329 .AAAGA..CC................A.... Ph Ch Se SA 1 TGGTATCAGAGCGATGTT 1 .................. 1 .................. 1 .................. 1 ............A..... Ph Ch Se Wb/Kk Ap/Pb SEA SEA SA b. Transcription Polyadenylation consensus TSS TATA box 7373 TATATAA—(N)24—TC—(N)192—AATAAA 7381 .......—(N)24—..—(N)193—...... 7381 .......—(N)27—..—(N)195—...... 7359 .......—(N)25—CA—(N)197—...... 7359 .......—(N)25—CA—(N)197—...... 7327 .......—(N)25—CA—(N)203—...... 7353 .......—(N)25—CA—(N)202—...... Ph Ch Se Wb Kk Ap Pb SEA SA c. Splicing Splice donor site Splice acceptor site Eukaryotic consensus Eukaryotic consensus Ph Ch Se Wb Kk Ap Pb 7495 ATGGCTCAGgtcagtgagtag —(N)6456— 5970 cagGGACA 7504 ..................... —(N)6460— 5975 ........ 7508 ..................... —(N)6457— 5970 ........ 7487 ..................... —(N)6398— 5972 ....ATA. 7487 ..................... —(N)6397— 5972 ....ATA. 7460 ..................... —(N)6398— 5942 ....ATA. 7486 ..................... —(N)6396— 5972 ....ATA. A/CAG GTPu AGT CAG G SEA SA sORF1 5’ of ORF IV Spliced Supplementary Fig. 1
