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PhD Course . TOPICS IN (NANO) BIOTECHNOLOGY Lecture I. 25th February, 2004. Overview . 1. What is Biotechnology? Definitions of Biotechnology Timeline of Biotechnology Techniques used in Biotechnology Who's Who in Biotechnology 2. How is Biotechnology being used?

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slide1

PhD Course

TOPICS IN (NANO) BIOTECHNOLOGY

Lecture I

25th February, 2004

slide3

Overview

  • 1. What is Biotechnology?
  • Definitions of Biotechnology
  • Timeline of Biotechnology
  • Techniques used in Biotechnology
  • Who's Who in Biotechnology
  • 2. How is Biotechnology being used?
  • Applications of Biotechnology
  • Medicines on the market today
  • Agriculture - GM Foods and Animals
  • DNA fingerprinting and forensic science
  • Gene Therapy and Transgenic Animals
  • Human Embryonic Stem Cells and Cloning
  • 3. What are some of the societal issues Biotechnology raises?
  • Bioethics / "Genethics"
  • Public attitudes to biotechnology - safety, awareness
  • Therapeutic uses of human genes and tissues
what is biotechnology
What is biotechnology?
  • Biotechnology = bios (life) + logos (study of or essence)
    • Literally ‘the study of tools from living things’
  • CLASSIC: The word "biotechnology" was first used in 1917 to describe processes using living organisms to make a product or run a process, such as industrial fermentations. (Robert Bud, The Uses of Life: A History of Biotechnology)
  • LAYMAN: Biotechnology began when humans began to plant their own crops, domesticate animals, ferment juice into wine, make cheese, and leaven bread (AccesExcellence)
slide5

What is biotechnology?

  • GENENTECH: Biotechnology is the process of harnessing 'nature's own' biochemical tools to make possible new products and processes and provide solutions to society's ills (G. Kirk Raab, Former President and CEO of Genentech)
  • WEBSTER’S: The aspect of technology concerned with the application of living organisms to meet the needs and ends of man.
  • WALL STREET:Biotechnology is the application of genetic engineering and DNA technology to produce therapeutic and medical diagnostic products andprocesses. Biotech companies have one thing in common - the use of genetic engineering and manipulation of organisms at a molecular level.
slide6

What is biotechnology?

  • Using scientific methods with organisms to produce new products or new forms of organisms
  • Any technique that uses living organisms or substances from those organisms or substances from those organisms to make or modify a product, to improve plants or animals, or to develop microorganisms for specific uses
slide7

What is biotechnology?

  • Biotechnology is a multidisciplinarian in nature, involving input from
    • Engineering
    • Computer Science
    • Cell and Molecular Biology
    • Microbiology
    • Genetics
    • Physiology
    • Biochemistry
    • Immunology
    • Virology
    • Recombinant DNA Technology  Genetic manipulation of bacteria, viruses, fungi, plants and animals, often for the development of specific products
slide8

What are the stages of biotechnology?

  • Ancient Biotechnology
    • early history as related to food and shelter, including domestication
  • Classical Biotechnology
    • built on ancient biotechnology
    • fermentation promoted food production
    • medicine
  • Modern Biotechnology
    • manipulates genetic information in organism
    • genetic engineering
slide9

Ancient biotechnology

History of domestication and agriculture

  • Paleolithic society – Hunter-gatherers  Nomadic lifestyle due to migratory animals and edible plant distribution (wild wheat and barley) (~2 x 106 yrs.)
  • Followed by domestication of plants and animals (artificial selection)  People settled, sedentary lifestyles evolved (~10,000 yrs. ago)
    • Cultivation of wheat, barley and rye (seed collections)
    • Sheep and goats  milk, cheese, button and meat
    • Grinding stones for food preparation
    • New technology  Origins of Biotechnology  Agrarian Societies
  • History of domestication and agriculture History of domestication and agriculture History of domestication and agriculture
slide10

Ancient biotechnology

Fermented foods and beverages

  • Long history of fermented foods since people began to settle (9000 BC) (fervere –to boil)
    • Often discovered by accident!
    • Improved flavor and texture
    • Deliberate contamination with bacteria or fungi (molds)
    • Examples:
      • Bread
      • Yogurt
      • Sour cream
      • Cheese
      • Wine
      • Beer
      • Sauerkraut
slide11

Ancient biotechnology

Fermented foods and beverages

  • Dough not baked immediately would undergo spontaneous fermentation  would rise  Eureka!!
  • Uncooked fermented dough could be used to ferment a new batch  no longer reliant on “chance fermentation”
  • 1866 – Louis Pasteur published his findings on the direct link between yeast and sugars  CO2 + ethanol (anaerobic process)
  • 1915 – Production of baker’s yeast – Saccharomyces cerevisiae
slide12

Classical biotechnology

Industry today exploits early discoveries of the fermentation process for production of huge numbers of products

  • Different types of beer
  • Vinegar
  • Glycerol
  • Acetone
  • Butanol
  • Lactic acid
  • Citric acid
  • Antibiotics – WWII (Bioreactor developed for large scale production, e.g. penicilin made by fermentation of penicillium)
    • Today many different antibiotics are produced by microorganisms
    • Cephalosporins, bacitracin, neomycin, tetracycline……..)
slide13

Classical biotechnology

Chemical transformations to produce therapeutic products

  • Substrate  + Microbial Enzyme  Product
  • Examples:
    • Cholesterol  Steroids (cortisone, estrogen, progesterone) (hydroxylation reaction  -OH group added to cholesterol ring)
slide14

Classical biotechnology

Microbial synthesis of other commercially valuable products

  • Amino acids to improve food taste, quality or preservation
  • Enzymes (cellulase, collagenase, diastase, glucose isomerase, invertase, lipase, pectinase, protease)
  • Vitamins
  • Pigments
slide15

Modern biotechnology

  • Cell biology
    • Structure, organization and reproduction
  • Biochemistry
    • Synthesis of organic compounds
    • Cell extracts for fermentation (enzymes versus whole cells)
  • Genetics
    • Resurrection of Gregor Mendel’s findings  1866  1900s
      • Theory of Inheritance (ratios dependent on traits of parents)
      • Theory of Transmission factors
    • W.H. Sutton – 1902
      • Chromosomes = inheritance factors
    • T.H. Morgan – Drosophila melanogaster
slide16

Modern biotechnology

  • Molecular Biology
    • Beadle and Tatum (Neurospora crassa)
      • One gene, one enzyme hypothesis
        • Charles Yanofsky  colinearity between mutations in genes and amino acid sequence (E. coli)
        • Genes determine structure of proteins
    • Hershey and Chase – 1952
    • T2 bacteriophage – 32P DNA, not 35S protein is the material that encodes genetic information
slide17

Modern biotechnology

  • Watson, Crick, Franklin and Wilkins (1953)
    • X-ray crystallography
    • 1962 – Nobel Prize awarded to three men
    • Chargaff – DNA base ratios
    • Structural model of DNA developed
  • DNA Revolution – Promise and Controversy!!!
  • Scientific foundation of modern biotechnology
    • based on knowledge of DNA, its replication, repair and use of enzymes to carry out in vitro splicing DNA fragments
slide18

Modern biotechnology

  • Breaking the Genetic Code – Finding the Central Dogma
  • An “RNA Club” organized by George Gamow (1954) assembled to determine the role of RNA in protein synthesis
  • Vernon Ingram’s research on sickle cell anemia (1956) tied together inheritable diseases with protein structure
    • Link made between amino acids and DNA
  • Radioactive tagging experiments demonstrate intermediate between DNA and protein = RNA
    • RNA movement tracked from nucleus to cytoplasm  site of protein synthesis
slide19

Modern biotechnology

  • DNA  RNA  Protein
    • TranscriptionTranslation
    • Genetic code determined for all 20 amino acids by Marshal Nirenberg and Heinrich Matthaei and Gobind Khorana – Nobel Prize – 1968
      • 3 base sequence = codon
slide20

What are the areas of biotechnology?

  • Organismic biotechnology
    • uses intact organisms and does not alter genetic material
  • Molecular Biotechnology
    • alters genetic makeup to achieve specific goals
  • Transgenic organism: an organism with artificially altered genetic material
slide21

What are the benefits of biotechnology?

  • Medicine
    • human
    • veterinary
    • biopharming
  • Environment
  • Agriculture
  • Food products
  • Industry and manufacturing
slide22

What are the applications of biotechnology?

  • Production of new and improved crops/foods, industrial chemicals, pharmaceuticals and livestock
  • Diagnostics for detecting genetic diseases
  • Gene therapy (e.g. ADA, CF)
  • Vaccine development (recombinant vaccines)
  • Environmental restoration
  • Protection of endangered species
  • Conservation biology
  • Bioremediation
  • Forensic applications
  • Food processing (cheese, beer)
slide23

Anti-cancer drugs

Transfer of new genes into animal organisms

Culture of plants from single cells

Diagnostics

Crime solving

Tracers

DNA technology

Genetic Engineering

Synthesis of specific DNA probes

Banks of DNA, RNA and proteins

Cloning

Synthesis of new proteins

Mass prodn. of human proteins

Localisation of genetic disorders

Resource bank for rare human chemicals

New types of plants and animals

New antibiotics

New types of food

Gene therapy

Cell

Culture

Monoclonal

Antibodies

Molecular

Biology

Complete map of the human genome

slide24

Biotechnology Timeline

1675 Leeuwenhoek discovers cells (bacteria, red blood cells)

1750 BC The Sumerians brew beer.

500 BC Chinese use moldy soybean curds as an antibiotic to treat boils

1590 Janssen invents the microscope

1830 Proteins are discovered

1833 The first enzymes are isolated

1855 The Eschirium coli bacterium is discovered

slide25

Biotechnology Timeline

1859 Charles Darwin publishes On the Origin of Species

1865 The age of genetics begins

1864 Louis Pasteur shows all living things are produced by other living things

1902 Walter Sutton coins the term ‘gene’ - proposed that chromosomes carry genes

slide26

Biotechnology Timeline

1910 Chromosomal theory of inheritance proved

1928 Fleming discovers antibiotic properties of certain molds

1941 George Beadle and Edward Tatum propose that one gene makes one protein

1949 Sickle cell anaemia demonstrated to be molecular disease

slide27

Biotechnology Timeline

1953 The double helix is unravelled

1973 Recombinant DNA technology begins

1952 The ‘Waring Blender’ experiment

1967 The genetic code is cracked

1975 First international conference on recombinant DNA technology

slide28

Biotechnology Timeline

1978 Kary Mullis discovers PCR

1975 DNA sequencing discovered

1975 Monoclonal antibody technology introduced

1978 Genentech Inc. established

1978 Genentech use genetic engineering to produce human insulin in E.coli - 1980 IPO of $89

slide29

Biotechnology Timeline

1994 The FLAVRSAVR tomato - first genetically engineered whole food

1989 The Human Genome Project begins

1990 First use of gene therapy

1990 First product of recombinant DNA technology introduced into US food chain

1993 FDA announces that transgenic food is safe

slide30

Biotechnology Timeline

1996 First mammal cloned from adult cells

1996 Development of Affymetrix GeneChip

1997 First artificial chromosome

1990s First conviction using genetic fingerprinting

slide31

History of Biotechnology

1999 Celera announces completion of Drosophilia genome sequence

2000 90% of Human Genome sequence published on web

2001 Human genome project complete

1998 Human embryonic stem cells grown

slide32

Excercise

  • Choose 3 scientists who have contributed to the biotechnology revolution and write a paragraph describing their input
  • Discover more about the Asilomar conference and decribe its signficance on the use of recombinant DNA technology
  • Discover more about what led to the death of the FLAVRSAVR tomato.
  • Follow one ‘linked set of discoveries’ outlining the path from the first experiment to today.
slide33

Excercise

  • Compare and argue both sides of Monsanto vs Greenpeace on the theme of genetically modified food
  • Outline the IMCLONE story and explain the potential impact on biotechnology industry.
  • Argue both for and against the use of human embryonic stem cells and outline international stance on this research.
  • Outline the story of the race to unravel DNA.
slide34

Discussion

  • What is the societal impression of biotechnology?
  • What are the negative impacts that biotechnology may have?
  • What are the potential ethical issues associated with biotechnology?
  • Why are biotechnology companies targeted by anti-globalisation protesters?
  • How can the image of biotechnology to the public be improved? Should it be improved?
  • What are the potential dangers of biotechnology?
slide35

Useful Resources

  • http://www.geocities.com/cwfennhcc/bi200/intro.html
  • http://www.geocities.com/cwfennhcc/bi200/quiz1.htm
  • http://www.accessexcellence.org/
  • The Uses Of Life – A History of Biotechnology (Robert Bud)