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Does spatial distribution of boutons relate to orientation preference in layer IV spiny neurons?

Does spatial distribution of boutons relate to orientation preference in layer IV spiny neurons?. Fuyuki KARUBE and Zoltán F. Kisvárday Laboratory for Cortical Systems Neuroscience, Medical and Health Science Center (MHSC), University of Debrecen, HUNGARY. Materials and Methods.

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Does spatial distribution of boutons relate to orientation preference in layer IV spiny neurons?

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  1. Does spatial distribution of boutons relate to orientation preference in layer IV spiny neurons? Fuyuki KARUBE and Zoltán F. Kisvárday Laboratory for Cortical Systems Neuroscience, Medical and Health Science Center (MHSC), University of Debrecen, HUNGARY

  2. Materials and Methods • Adult cats (2.3 - 3.2 kg) were used. • Visual stimuli Moving luminance gratings (square waves; frequency, 0.1-0.8 cpd; velocity, 2 Hz). • Imaging Cortex (A18) was illuminated at 605 nm light. Intrinsic signals were captured with a CCD camera. • Extracellular recordings to identify receptive field properties. • Tracer injections for extracellular staining • Reconstruction of stained single cells by Neurolucida.

  3. Activity dependent changes in [oxyHb] and [deoxyHb] Arbitrary units Time after stimuli onset (s) Time course of intrinsic signal Start visual stimulation Stop stimulation From Grinvald and Bonhoeffer

  4. Avtivated area by vertical gratings Optical imaging of intrinsic signal From Grinvald and Bonhoeffer

  5. Analysis of map structure for orientation preference

  6. Orientation preference patches elongated along iso-elevation line in visual field

  7. Reconstructions of layer IV spiny neurons Dendrites were drawn in black, axons in red.

  8. Bouton distribution pattern relative to orientation preference Local Within 400mm diameter Distal

  9. Bouton distribution in iso-,oblique-, and cross-orientation patches

  10. Tentative LMand AP-elongated cells on orientation preference map

  11. Quantification of bouton distribution

  12. Bouton distribution to cortical dimensions and preferred orientations

  13. conclusion • Layer IV spiny neurons showed various bouton distribution from cell to cell. half of them preferred iso-orientation, another half did cross-orientation. • Orientation patches elongated with LM (or iso-elevation) axis. Thus, spatial distribution of boutons of single cells can affect connectivity between iso- or cross-orientation preference patches.

  14. Coronal view Tangential view

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