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Platelet Function Testing

Platelet Function Testing. Kit for each patient The packet contains everything needed. We will now demonstrate how to perform the test. Three platelet stimulant tubes. Instruction leaflet Citrate blood collection tube. aspirin test clopidogrel test baseline test. insulated pouch

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Platelet Function Testing

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  1. Platelet Function Testing Kit for each patient The packet contains everything needed We will now demonstrate how to perform the test

  2. Three platelet stimulant tubes Instruction leaflet Citrate blood collection tube aspirin test clopidogrel test baseline test insulated pouch heating pad connecting 2way adaptors fixing solution

  3. Warming the tubes Activate the heating pad by clicking on the circular valve inside

  4. Warming the tubes When activated the gel turns white and warms up immediately

  5. Warming the tubes Place the heated pad inside one pocket of the insulation pouch

  6. Warming the tubes Place the three stimulant tubes in the other pocket of the insulation pouch

  7. Warming the tubes The stimulant tubes are now ready to use

  8. Venepuncture Select the green topped citrate monovette Blood should preferably be collected using one of the following venepuncture procedures Butterfly BD Vacutainer Sarstedt Multifly set

  9. Venepuncture Butterfly you will also need the Sarstedt multi-adaptor valve

  10. Venepuncture BD Vacutainer Safety-Lok you also need the Sarstedt multi-adaptor valve

  11. Venepuncture the Sarstedt multi-adaptor valve

  12. Venepuncture Sarstedt MultifySet already supplied with the adaptor for the monovette tube

  13. Filled citrate anticoagulant tube Fill to the 10 ml line by pulling gently on the plunger (do not lock off the tube) Invert 3 times to mix the blood

  14. Venepuncture If you have to use a needle and syringe If using a needle and syringe the tube will need to be opened by holding the monovette upright and unscrewing the green end taking care not to spill the anticoagulant inside. 10 ml mark Pull the plunger pulled back (do not lock off the tube) Add the blood from the syringe up to the 10 ml mark Screw on the green cap Invert 3 times to mix the blood

  15. Filled citrate anticoagulant tube

  16. Place the blood in the insulation pouch with the stimulant tubes until ready to perform the test

  17. Platelet stimulation Mix the blood and attach one of the 2way adaptors

  18. Platelet stimulation Fill three stimulant tubes Attach each stimulant tube and add blood in turn

  19. Platelet stimulation Blood should flow up to 4.5 ml mark To fill, pull back plunger until top of the plunger aligns with bottom arrow

  20. Platelet stimulation Place the stimulant tubes back in the pouch to keep warm Check the time and wait 5 minutes!

  21. While waiting for the 5 minutes Discard the used citrate tube and adaptor valve

  22. While waiting for 5 minutes Take the second 2way adaptor and attach to the Fixing Solution Tube

  23. When 5 minutes is passed Attach each stimulant tube in turn to the 2way adaptor Pull back plunger all the way and fill the tubes with the fixing solution (liquid aligns with the 4.5 ml mark)

  24. Completed stimulant tubes Invert to mix

  25. Snap off the plungers on the stimulant tubes

  26. Write patient details on the labels on each of the three stimulant tubes

  27. Place the completed stimulant tubes in the padding for transportation

  28. Place tubes inside the package and transfer pack to Nottingham Platelet function will be measured as platelet P-selectin expression

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