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ABSTRACT Total Isolation Of Mice Testis ( Mus musculus. L) With Analyze Spectrofotometry UV and Electroforesis (Comparing Method : Trozol TM , Guanidium thiosyanat, Guanidium thiosyanat-CsCl) Mutia Widiyani This aimof the research was to compareing the three method of total RNA.

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ABSTRACT

Total Isolation Of Mice Testis (Mus musculus.L) With Analyze

Spectrofotometry UV and Electroforesis (Comparing Method : TrozolTM,

Guanidium thiosyanat, Guanidium thiosyanat-CsCl)

Mutia Widiyani

This aimof the research was to compareing the three method of total RNA

isolation by reagen TrizolTM

Guanidium Thiocyanat,

and Guanidium

ThiocyanatCsCl. From those methods will be discovered which one of those methods

is giving highest quality and quantity of RNA. This research is using mice testis (Mus

musculus. L) as it materials and the result of RNA total isolation achieved was anlyze

using spectrophotometri with λ max 260 nm, for absorbant analysis with λ max 260

nm the quantity of RNA, while to see the purity measurement can be achived from

with comparison 260/280 nm. To see its quality profile electrophoresis with 1%

agarose gel. From three methods isolation of total RNA that is by using TrizolTM

result in total isolation of total RNA 0,8202 μg/μL/mg.

Key word: RNA Total isolation, TrizolTM Guanidium Thiocyanat, and

Guanidium Thiocyanat-CsCl