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  1. LONGEVA project Clubof Experimental Sciences EB 2,3/S Dr. Daniel de Matos School, Vila Nova de Poiares, Portugal Pinhal do Rei, SecondarySchool, Marinha Grande, Portugal

  2. LONGEVA Project CV - 1825 ► Pedagogicandscientificproject ► Clubof Experimental Sciences ► Project Methodology Firstresults: Poster publishedin a InternationalScientificCongress (U.S.A)

  3. Scientific Method - Problem-Based Learning Students had: proposed problem looked for information formulated hypotheses made experiments analysed results divulged scientific discovery

  4. initialproblem Itispossible to extendhumanlifespan ?

  5. theoreticalinquiry ► Longevity has a strong genetic component ► Some foods seem to be associates to the longevity ► Caloric restriction slows aging ► Some biomolecules can activate the sirtuins (genes of “immortality”) and extend lifespan

  6. Resveratrol ► Vegetal origin Polygonum cuspidatum ► Extends life span in many different organisms, including mammals ► Therapy of aging pathologies: Cardiovascular Diseases Cancer Parkinson Alzheimer

  7. Resveratrol ► Extends Lifespan of Yeast by 70% ► Extends Lifespan of fish by almost 60 % ► Extends Lifespan of Fat Mice

  8. Yeast - Saccharomycescerevisiae ideal biologicmodel Leaven of Baker ► eucariotic microorganism ► short duration of life ► easy maintenance in laboratory ► it does not raise ethical questions ► it does not have risks for the young students

  9. Hypothesis If the longevity is correlates with the resistance to oxidative and thermal stresses, then… it will be that resveratrol increases the resistance to thermal stress in yeast? If we confirmed this hypothesis a rapidtest for theidentificationofotherslifespan-extendingbiomolecules

  10. Materials e Methods + Yeastculturemedium : + + Y C M 1 g of Leaven 100 ml of Water

  11. Experience (step 1) – oxidative stress Control Tube Experience Tube 100 µl YCM + 100 µlwater + 100 µlMB* 100 µl YCM + 100 µl of resveratrol+ 100 µlMB* MB* – MethyleneBlue(after 1 minute)

  12. Experience (step 2) – thermal stress Control Tube Experience Tube 30 seconds in Microwaves (900 W)

  13. Observationinopticmicroscope

  14. Countingcells Live yeast Dead yeast

  15. Results Control 100% Resveratrol 60% mortality

  16. Implications ► Resveratrolincreasesoxidativeandthermalstressesresistence ► Inonlyabout 2 minutes wecouldtestthousandsofvegetableextracts ► Discoveryofbiomolecules more potentsthanresveratrol

  17. Project impact Firstresults Poster publishedin a InternationalScientificCongress (U.S.A)

  18. Project impact ► Portuguese television, news: RTP (Portugal directo – 23 June 2006) ► Science program in Portuguese television: RTP (ABCiência) http://www.youtube.com/watch?v=-EGv5iT53nM&feature=related ► 5 times in national press ► Presented at Science teaching festival, Grenoble, France, 2-6 April 2007

  19. Newinvestigationlines ► test several plant extracts with medical effects ► test vegetable extracts of trees with great longevity

  20. Newresults Did we find the elixir of immortality? Without the elixir (100% dead cells) With the elixir (100% live cells)

  21. Isimmortalitypossible? Dr. Alexis Carrel (1873-1944), the scientist who won the Nobel prize for keeping the cells of a chicken heart alive for 34 years stated: "The cell is immortal. It is merely the fluid in which it floats that degenerates. Renew this fluid at intervals, give the cells what they require for nutrition and, as far as we know, the pulsation of life may go on forever.”

  22. Firstpublication The Free Encyclopedia • Elixir of life • Life extension

  23. Acknowledgements: Entidades e Instituições Project supported by EB 2,3/S Dr. Daniel de Matos School, Vila Nova de Poiares Todos os alunos e professores que colaboraram na elaboração do poster Secondary School / 3 Pinhal do Rei, Marinha Grande