4A, B, C, D Timeline. Monday, October 1 st DNA & RNA Lecture Tuesday, October 2 nd Pre-Lab Quiz, Lab 4A (if time) Thursday, October 4 th Lab 4B (50 minutes) Monday, October 8 th MAGIC MOUNTAIN! Tuesday, October 9 th Step 12 from Lab B, Lab 4C Thursday, October 11 th
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Check supplies: sodium chloride, TRIS, EDTA, pH paper, HCl & NaOH
Check supplies: salmon sperm DNA,
Check supplies: salmon sperm DNA, gelatin, DPA, sulfuric acid, acetic acid, acetaldehyde, NaOH 10%, Cupric Sulfate 5%
Prepare NaOH 10% and Cupric Sulfate
Dilute ethidium bromide
Making Solutions for DNA Isolation
Show calculations involved in lab notebook
Draw diagram of how solution is prepared in notebook
Store finished mixture at 4 degrees Celsius until ready for use
Students who finish early should make extra TE buffer for later use?
Analytical balances may be used for TRIS and EDTA measurements
5 M NaClis very concentrated; it may take a long time for the sodium chloride to fully dissolve.
Pulling DNA out of Solution: DNA Spooling
Testing for the Presence of DNA, RNA, and Protein in DNA Extracts
DPA produces toxic fumes and should only be used in a chemical fume hood with a fan running
Poor spooling technique may contaminate DNA sample with alcohol, which will yield inaccurate results
EtBR Dot Test: A Quick Test for DNA in Samples