Loading in 2 Seconds...
Loading in 2 Seconds...
Statistical Methods for Rare Variant Association Test Using Summarized Data. Qunyuan Zhang Ingrid Borecki, Michael A. Province Division of Statistical Genomics. Next generation sequencing => rare variants Two types of data. Motivation. Summarized level. Pooled DNA sequencing
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Statistical Methods for Rare Variant Association Test Using Summarized Data Qunyuan Zhang Ingrid Borecki, Michael A. Province Division of Statistical Genomics
Next generation sequencing => rare variants Two types of data Motivation Summarized level • Pooled DNA sequencing • Public data (as control) Individual level
EFTTFT QQ Plots of Existing Methods(under the null) EFT and C-alpha inflated with false positives TFT and CAST no inflation, but assuming single effect-direction Objective More general, powerful methods … CAST C-alpha
variant 1 variant 2 … … variant 3 variant k variant i Structure of Summarized data Strategy Instead of testing total freq./number, we test the randomness of all tables.
Exact Probability Test (EPT) 1.Calculating the probability of each table based on hypergeometric distribution 2. Calculating the logarized joint probability (L) for all k tables 3. Enumerating all possible tables and L scores 4. Calculating p-value P= Prob.( )
Likelihood Ratio Test (LRT) Binomial distribution
EPT N=500 LRT N=500 Q-Q Plots of EPT and LRT(under the null) LRT N=3000 EPT N=3000
Power Comparison significance level=0.00001 Variant proportion Positive causal 80% Neutral 20% Negative Causal 0% Power Power Power Sample size Sample size Sample size
Power Comparisonsignificance level=0.00001 Variant proportion Positive causal 60% Neutral 20% Negative Causal 20% Power Sample size
Power Comparison significance level=0.00001 Variant proportion Positive causal 40% Neutral 20% Negative Causal 40% Power Sample size
Power Comparison individual-level data vs. summarized dataN=1000, significance level=0.00001 Power CMC Li & Leal, 2008 SKAT Wu et al., 2011 Variant proportion positive : neutral : negative (%)
Cases: 460 ovarian cancer cases, germline exome data, from TCGA Controls: ~3500 individuals, exome data, from NHBLI Application -LOG10 p-values of 933 cancer-related genes
Conclusions EFT and C-alpha produce inflated p-value. TFT and CAST produce correct p-value, but lose power in detecting bi-directional effects. EPT produces correct p-value and maintains power regardless of effect directions, more computer time. LRT produces slightly biased p-value for small N, can be improved by larger N, similar power of EPT, less computer time, a good alternative for large datasets. If no confounders need to be modeled, there is no significant loss of power in the use of summarized data
Acknowledgements Dr. Li Ding Charles Lu Krishna-Latha Kanchi (for providing the TCGA and NHBLI exome data)