Molecular genetics of bacteria. Emphasis: ways that bacteria differ from eukaryotes DNA structure and function; definitions. DNA replication Transcription and translation Gene regulation and regulation of metabolism Genetic exchange among bacteria. DNA structure. double helix
Because of requirement for 5’to 3’ synthesis, lagging strand must repeatedly top and start; needs an RNA primer each time.
1) DNA is double stranded, but RNA is single stranded.
However, RNA can base-pair with itself to create double stranded regions.
2) RNA contains ribose instead of deoxyribose
3) RNA contains uracil instead of thymine.
mRNA: a copy of the gene; is translated to make protein.
tRNA: smallest RNA, does actual decoding.
rRNA: 3 sizes that, along with proteins, make up a ribosome.
Tobin and Duschek, Asking About Life; http://www.tokyo-ed.ac.jp/genet/mutation/nort.gif