Molecular genetics of bacteria. Emphasis: ways that bacteria differ from eukaryotes DNA structure and function; definitions. DNA replication Transcription and translation Gene regulation and regulation of metabolism Genetic exchange among bacteria. DNA structure. double helix
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Because of requirement for 5’to 3’ synthesis, lagging strand must repeatedly top and start; needs an RNA primer each time.
1) DNA is double stranded, but RNA is single stranded.
However, RNA can base-pair with itself to create double stranded regions.
2) RNA contains ribose instead of deoxyribose
3) RNA contains uracil instead of thymine.
mRNA: a copy of the gene; is translated to make protein.
tRNA: smallest RNA, does actual decoding.
rRNA: 3 sizes that, along with proteins, make up a ribosome.
Tobin and Duschek, Asking About Life; http://www.tokyo-ed.ac.jp/genet/mutation/nort.gif