Sputum Culture and Throat Swab. Aim of the test. An etiological diagnosis of lower respiratory tract infection by microscopic examination and culture with identification and susceptibility test of the isolated organism. Types of specimen
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Selective media which selects for Gram-positive bacteria.
It contains two antibiotics, colistin and naladixic acid
Streak the swab across blood agar plate and Columbia CNA to make a line that divide the plate into two halves, and using a sterile loop, streak by crossing the line to produce isolated colonies. Make few stabs in the agar.
Do a gram stain from the swab noting the predominant organism.
Rheumatic fever remains a concern all over the world and serious complications including sepsis, soft tissue invasion, and toxic shock-like syndrome have been reported to be increasing in frequency; therefore, timely diagnosis and early institution of appropriate therapy remains important.