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Immunological characteristics of patients infected with common intestinal helminths : result of a study based on reverse-transcriptase PCR S.Lertanekawattana,T.Wichatrong,K.Chaisari, R.Uchikawa and N.Arizono

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slide1
Immunological characteristics of patients infected with common intestinal helminths : result of a study based on reverse-transcriptase PCR

S.Lertanekawattana,T.Wichatrong,K.Chaisari, R.Uchikawa and N.Arizono

Nongkhai Hospital and Department of Medical Zoology,

KyotoPrefectural University of Medicine

slide3
Long-term infection intestinal parasites :

-Malnutrition

-Retardation of the intelectual

-Impair physical growth of children

Oberhelman et al.,1998:

slide4
Helminth infection :

-Impair immune response to virus,bacteria protozoa and even oral vaccine

( Cooper et al, 2001)

slide5
Ascaris and Hook worm induce expansion of the Th2 lymphocyte subset and regulate IgE antibody production, eosinophilia and mastocytosis

(Pritchard et al,1995)

slide6
Th2 cytokines have antagonistic effects on the expansion of the Th1 lymphocyte subset

(Cooper et al,2001)

slide10
Anisakiasis

Clinical : Urticaria,Angioedema,

Anaphylaxis

Immunological response :

IgE , mast cell, basophil

Th 2 interleukin 4,predominant

slide11
Villus pathology induced by the rodent nematode Nippostrongylus brasiliensis
  • Nippostrongylus brasiliensis
slide12
4

3

2

1

E-cadherin expression decreases at villus tips after infection

Day 0

Day 10

E-cadherin score

0

28

7

10

14

days after infection

E-cadherin immunohistochemistry

slide13
Pathological changes develop in the intestinal mucosa,including partial villus atrophy and crypt hyperplasia,decreasing of sucrase and alkaline phosphatase activity,deterioration of epithelial permeability and barrier function

(Hyoh et al,2002 ; Sekikawa et al;2003)

slide14
Glucose transport in the small intestine

Lumen

SGLT1 (Na-driven glucose cotransporter)

glucose

Na

*

*

Absorptive

cell

glucose

Na

K

Na-K ATPase

Lamina propria

glucose

Na

slide15
Infection with intestinal nematodes in rodents causes induction Th2 cytokines such as IL-4.IL-5and IL-13 and induce IgE antibody responses, eosinophilia and intestinal mastocytosis

(Matsuda et al, 1995 ; Garside et al,2000)

slide16
Aim of study :

To determine whether common helminth infections could modify the intestinal immunopathological status of the host,by the expression in the human duodenal mucosa of cytokines,eosinophil,mast cell specific molecule,monosaccharide transporters of the glucose-transporter (GLUT)

slide17
Subjects :

31 patients with gastrointestinal problems and indicated for gastroduodenal fiberscopy as diagnotic measurement and did not have dysenteric disease and gave their written,informed consent for the study

slide18
Materials:

Duodenal biopsy specimens

slide19
Methods :

Semi-quantitative RT-PCR for IL-5 ,IL-13, IFN- , GLUT-1 and GLUT-5

slide20
Laboratory study:

At central lab. of Kyoto Prefectural University of Medicine

slide21
Density analysis of PCR products

Electrophoresis on agar each band was evaluated using NIH Image software, the band densities were normalized relative to those of ß-actin

slide25
Figure 1 Some of PCR results,showing the expression of IL-13,IL-5,IFN-,andfor reference,-actin in the duodenal mucosa of the 31 patients
slide26
Figure 2 (a) The frequencies of expression of IL-5( )and IL-13 ( )

In the duodenal mucosa of the 10 patients with eosinophilia and the 21 without

slide27
Figure 3 (b) In the 9 patients with helminth infection(on presentation or in the previous 3 months ) and the 22 without

P=0.041

slide30
Summary:

The present findings indicate that infections with common intestinal helminths, including Taeniasis are likely to modify mucosal immunology so that it shifts to a Th2-predominant status.

SGUT-1 and GLUT-5 no significant decrease in patients with helminth infection

slide31
Acknowledgment:

This work was supported by Grants-in-Aid for Research from the Japan Society for the Promotion of Science and the Japanese Ministry of Health,Labour and Welfare

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