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A metabolite-binding ‘aptamer’ domain and …. … an ‘expression platform’ for gene regulation. Riboswitch Functions. Direct (protein-free) binding of metabolite to RNA Metabolite-dependent regulation of genes.

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Presentation Transcript
slide9

A metabolite-binding

‘aptamer’ domain

and …

… an ‘expression platform’ for gene regulation.

Riboswitch Functions

  • Direct (protein-free) binding of metabolite to RNA
  • Metabolite-dependent regulation of genes

These two activities are accomplished by two functionally separate domains on the RNA:

X

1

2

3

4

U

U

U

U

U

Courtesy: Ron Breaker Lab (Yale University)

slide10

X

ORF

A

U

G

X

GUACGG

AG

ORF

A

U

G

X

ORF

A

A

A

A

A

A

U

G

Riboswitch Mechanisms

They also affect RNA integrity, and perhaps splicing and stability.

Ligand binding leads to

mRNA cleavage by a new natural ribozyme.

X

Ligand binding could control

splicing in eukayotes.

Possibly, ligand binding to the 3´ untranslated region could affect mRNA stability.

Courtesy: Ron Breaker Lab (Yale University)

slide11

Riboswitch Ligands

There are 8 confirmed riboswitches with unique metabolite ligands.

Many more conserved RNA motifs are currently under investigation.

Courtesy: Ron Breaker Lab (Yale University)