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Molecular Cell, Volume 7, Issue 1 , 1 January 2001, Pages 43-54 PDB ID:1G5H

Crystal Structure and Deletion Analysis Show that the Accessory Subunit of Mammalian DNA Polymerase γ, PolB, Functions as a Homodimer. Molecular Cell, Volume 7, Issue 1 , 1 January 2001, Pages 43-54 PDB ID:1G5H. 作者簡介.

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Molecular Cell, Volume 7, Issue 1 , 1 January 2001, Pages 43-54 PDB ID:1G5H

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  1. Crystal Structure and Deletion Analysis Show that the Accessory Subunit of Mammalian DNA Polymerase γ, PolB, Functions as a Homodimer Molecular Cell, Volume 7, Issue 1 , 1 January 2001, Pages 43-54 PDB ID:1G5H

  2. 作者簡介 • 作者一覽：Jose A. Carrodeguas*, Karsten Theis^*, Daniel F. Bogenhagen*, and Caroline Kisker^* • *紐約州立大學石溪分校，藥理科學系。 • ^結構生物學中心。 • Caroline Kisker，美國紐約州立大學石溪分校，藥理科學系副教授。

  3. Main Biological Question • 已知PolγB會形成Dimer的型式與PolγA結合，組成Polγ holoenzyme，作為ATP-dependent processivity factor 。然而其dimerization與DNA binding motif到目前還是沒有人研究過。本篇作者便是為了解答上述的問題，而解出PolγB的結構。

  4. Results • 決定了mouse PolγB的結構 • PolγB具有類似於Glycyl-tRNA syhthase的folding方式，且有著額外的Dimerization Domain • 找到DNA binding site

  5. Dimerization Domain

  6. What I interesting • PolγB 的DNA binding motif與Glycyl-tRNA syhthase在立體結構上如此相似，是否表示了不同酵素的DNA binding motif在演化上有親緣關係？ • DNA polymerase是無論各種生物都有的，為了能達到high processive，幾乎所有的replicase都有processivity factor。但PolγB與PCNA之間，作用機制卻完全不相同，是否可為內共生說之證據？ • 或許能採用3D結構比對配合序列比對進行演化親緣關係的研究。

  7. 預期結果 • 配合3D結構比對與序列比對，透過演算法(如果有的話)計算出各種具有相似DNA binding motif的蛋白質間的distance，藉以描繪出DNA binding protein的樹狀關係圖，配合分子時鐘，或許找到並描繪出最早的protein-DNA interaction的情景。

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