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chapter five: microbial metabolism

chapter five: microbial metabolism

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chapter five: microbial metabolism

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  1. chapter five:microbial metabolism

  2. oxidation-reduction redox reaction: coupled reactions e- removed as part of H atom

  3. redox reactions aerobic respiration oxygenic photosynthesis

  4. carbon source organic vs. CO2 heterotroph autotroph energy source light/chemical energy source light/chemical chemoautotroph photoheterotroph chemoheterotroph photoautotroph electron source organic/inorganic electron source organic/inorganic electron source organic/inorganic electron source inorganic chemoorgano- autotroph chemolitho- autotroph photoorgano- heterotroph photolitho- heterotroph photolitho- autotroph chemoorgano- heterotroph chemolitho- heterotroph O2 vs. inorganic electron acceptor organic/inorganic fermentation respiration O2 vs. other oxygenic photosynthesis anoxygenic photosynthesis aerobic respiration anaerobic respiration nutritional classification: metabolic strategy sulfur oxidizers iron oxidizers lactic acid alcoholic mixed acid butanediol nitrate carbonate sulfate

  5. classifying respiration & photosynthesis

  6. complementary metabolism autotrophy heterotrophy

  7. acquiring energy: substrate level phosphorylation

  8. acquiring energy: oxidative phosphorylation & chemiosmosis

  9. C6H12O6 NAD+ ETC NADH CO2 ADP + P ATP 2 H+ heterotrophy: respiration NO2-, N2 + H2O H2S + H2O CH4 + H2O H2O NO3- SO4- CO32- O2 cell material

  10. C6H12O6 C6H12O6 NAD+ NAD+ ETC NADH NADH CO2 substrate P few ATP pyruvate ADP + P lots of ATP 2 H+ heterotrophy: respiration & fermentation reducede- acceptor inorganic e- acceptor lactic acid ethanol & CO2 mixed acids butanediol organic pyruvate ferm cell material

  11. heterotrophy: respiration & fermentation respiration • inorganic e- acceptor • does NOT mean O2 • organic mole.  CO2 fermentation • organic e- acceptor • organic  organic mole. • incomplete H stripping,lower ATP yield

  12. metabolism & media

  13. Chapter Five Learning Objectives • Discuss redox reactions in biological systems. • Identify the redox partners in aerobic and anaerobic respiration and oxygenic and anoxygenic photosynthesis. • Correctly identify the carbon, energy and electron source for an organism when given its nutritional classification (e.g., chemoorganoheterotroph). • How is ATP generated in both substrate level and oxidative phosphorylation? • Why is it so important that the electron transport chain is housed in a lipid bilayer membrane? Why is a terminal electron acceptor so important? • What happens in a microorganism if the terminal electron acceptor of the ETC is not available? What molecules build up? What is done with these molecules? • How do amphibolism, catabolism and anabolism relate to growth and repair in cells? • Discuss the major differences between respiration and fermentation. What are the four basic kinds of fermentation?

  14. chemo-: conversion of chemical E  ATP sulfur oxidation iron oxidation -synthesis: carbon fixation (CO2 organic molecule) autotrophy: chemosynthesis NAD+ NADH NAD+ NADH ETC ETC carbon fixation carbon fixation ADP + P ADP + P ATP ATP 2 H+ H2S SO42- 2 H+ 2Fe2+ 2Fe3+

  15. chemosynthesis: iron oxidation Thiobacillus ferrooxidans chemolithoautotrophy • energy = Fe2+ Fe3+ • electron = same • carbon = CO2 CH2O

  16. chemosynthesis: sulfur oxidation Sulfolobus acidocaldarius chemolithoautotrophy • energy = S2- (sulfide) / S2O32- (thiosulfate)  SO32- (sulfite) • electron = same • carbon = CO2 CH2O

  17. autotrophy: photosynthesis photo: light E  chemical E light-dependent (light) reactions ATP & NAD(P)H “reducing power” synthesis: light-independent (dark) reactions carbon fixation: piling e- onto CO2 NAD(P) NAD(P)H chlorophyll ETC carbon fixation (light independent reactions) oxidized chlorophyll ADP + P ATP H2S/H2O

  18. photosynthetic electron flow & chemiosmosis cyclic photosynthesis in the purple sulfur bacteria non-cyclic photosynthesis in the cyanobacteria

  19. microbial CO2 fixation

  20. photosynthesis compared [O2] [H2S]

  21. chapter 5 learning objectives • How is ATP generated in chemosynthesis, photosynthesis and respiration? How is the process different for each and how is it the same? • Discuss the redox partners of sulfur and iron oxidizing bacteria. • How do non-cyclic and cyclic photosynthesis differ? How does each produce ATP and NADPH/NADH? What is each used for? • How is carbon fixed during chemosynthesis and photosynthesis? How is the process similar and how is it different?

  22. chapter five:microbial metabolism