Protein enhanced bone recovery
1 / 14

Protein Enhanced Bone Recovery - PowerPoint PPT Presentation

  • Uploaded on

Protein Enhanced Bone Recovery. By Amanda Walker Working with Ashley Aston Weiner Mentored by Prasad Shastri. Analysis- Primary Objective. Main goal- to optimize protein release from a bone repair biopolymer Goal components minimize burst effect by optimizing porosity

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
Download Presentation

PowerPoint Slideshow about ' Protein Enhanced Bone Recovery' - ponce

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Protein enhanced bone recovery

Protein Enhanced Bone Recovery

By Amanda Walker

Working with Ashley Aston Weiner

Mentored by Prasad Shastri

Analysis primary objective
Analysis- Primary Objective

  • Main goal- to optimize protein release from a bone repair biopolymer

  • Goal components

    • minimize burst effect by optimizing porosity

    • Obtain a linear release of protein over 3 to 4 weeks (life span of polymer)

  • Factors to vary

    • Porogen material

    • Porogen concentration

    • Size of gelatin


Analysis problem statement
Analysis- Problem Statement

  • The data we have for the porosity effects are not sufficient.

  • Porogens mixed into polymer control protein release.

  • Previous protein release studies done from linear polymers.

Analysis polymer explanation
Analysis- Polymer explanation

How does it work?

  • Polyanhydride degrades over the course of a month

  • Porogens quickly dissolve creating holes

    • Exposes proteins

    • Proteins are growth factors

  • Bone grows in as material degrades


    Market analysis
    Market Analysis

    • The market

      • Broken bones

        • 3 femur fractures per 10,000 population annually for young and old people

    • Bone disease

      • 50% of women 50+ have fractures related to osteoporosis

  • Spinal fusion

    • 290,000 surgeries in 2003


    Market analysis1
    Market Analysis

    • Competition

      • ETEX corporation

        • Endothermic curing takes 15-20 minutes

        • No proteins

    • NovaBone

      • Molds to shape of injury, but not immediately

  • Benefits over competition

    • Less invasive

    • More comfortable

    • Custom fit

    • Instant curing

    • Enhancement by growth


    • Crosslinking=material



    Analysis background
    Analysis- Background

    • Precedent- BCNU release study

      • Wafer placed in hole after glioblastoma removal

      • Release of BCNU kills leftover cancer cells

      • Improves lifespan

    • This device is currently being used in medicine.

    • Several other papers were researched.

    Analysis material components
    Analysis- Material Components


    Porogens and Proteins

    • Monomers

      • MSA

      • MCPH

    • Porogen

      • Salt

      • Gelatin

    • Protein/sugar

      • HRP as test protein

    • Crosslinking initiator





    Blue light

    Crosslinked polymer

    Analysis polymerization
    Analysis- Polymerization

    • Initiator= camphorquinone

    • Forms free radicals when exposed to blue light

      • By hydrogen atom extraction

  • Radicals initiate crosslinking


    Synthesis of samples block diagram
    Synthesis of Samples- Block Diagram

    Mix protein and sugar

    Coomassie assay

    Granulate protein

    Mix monomers, protein, and porogen

    Make and/or quantify porogen

    Add initiator

    Mold and expose

    Hypothesis performance metrics
    Hypothesis- Performance Metrics

    How will improvement be measured?

    • Polymerized samples will be degraded in 37 degree PBS.

    • PBS will be changed daily.

    • The old PBS will be analyzed using a protein quantification assay.

    • This will determine how much protein was released over the last time period.

    Analysis performance criteria
    Analysis- Performance Criteria

    • Constraints

      • Release=3-4 weeks

      • Avoid burst effect

    • Limitations

      • Time- 4 months

      • Money- no real constraints

    • Exclusions- some porogens

      • FITC-dextran, for example

      • BSA as a protein- too much background in negative controls

    Burst Effect

    Validation current status
    Validation- Current Status

    • Accomplished:

      • Protein uniformity verification

      • Protein quantification

      • Protein and porogen granulation

      • Made the cylindrical samples for the first porogen (NaCl)

  • Current work

    • Had to change the protein used from BSA to horseradish peroxidase (HRP)

      • Due to this we are using a different assay to quantify release in the degradation studies

      • The Coomassie assay was still used for protein uniformity verification

    • Degradation study of the NaCl porogen samples

      • Slightly delayed (about 2 weeks) due to the necessity of changing the protein

  • ad